EMF-Portal - Absence of genotoxicity in human blood cells exposed to 50 Hz magnetic fields as assessed by comet assay, chromosome aberration, micronucleus, and sister chromatid exchange analyses (2004)

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Medical/biological Study (experimental study)

Absence of genotoxicity in human blood cells exposed to 50 Hz magnetic fields as assessed by comet assay, chromosome aberration, micronucleus, and sister chromatid exchange analyses. med./biol.

By: Stronati L, Testa A, Villani P, Marino C, Lovisolo GA, Conti D, Russo F, Fresegna AM, Cordelli E
Published in: Bioelectromagnetics 2004; 25 (1): 41 - 48 ( open external web page

PubMed Entry , open external web page

Journal web site )

Aim of study (according to author)
To evaluate the effects of 50 Hz magnetic fields in human blood cells exposed in vitro. The effects of a combined exposure with X-rays were also investigated.

Endpoint

genotoxicity/mutation: DNA damage

Exposure
General category: magnetic field, 50/60 Hz (AC)

Field characteristics	Parameters
50 Hz exposure duration: continuous for 2 h	magnetic flux density: 1 mT (75 V/m)

FIELD View further expo parameters

Exposed system:
blood samples

Methods
Endpoint/Measurement parameters/Methodology

genotoxicity/mutation: DNA damage (alkaline comet assay (tail moment: tail length x fraction of DNA in the tail; % tail DNA); cytogenetic assay, sister chromatid exchange and chromosome aberration (metaphase analysis); micronucleus test (frequency of micronuclei; Giemsa stain))
cell viability/cell division/proliferation: nuclear division index; proliferation index (proportion of cells that undergoes 1, 2, 3, or more divisions; metaphase analysis)

investigated material: DNA/RNA (in vitro), intact cell/cell culture (in vitro)

time of investigation: after exposure

Main outcome of study (according to author)
Data obtained do not show any significant difference between extremely low frequency magnetic fields exposed and unexposed samples. Moreover, no synergistic effect with ionizing radiation has been revealed. A slight but significant decrease of cell proliferation was evident in extremely low frequency magnetic fields treated cell samples and cell samples subjected to the combined exposure.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

Ministero dell'Ambiente e della Tutela del Territorio e del Mar (Ministry of Environment and Territory), Italy

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Glossary: 50 Hz, AC, alkaline, assay, biological, blood, blood cells, cell, cell culture, cell division, cell proliferation, cell viability, chromosome aberration, comet assay, cytogenetic, DNA, endpoint, evident, exposed, exposure, extremely low frequency, frequency, full/main study, genotoxicity, Giemsa stain, human, in vitro, ionizing radiation, magnetic fields, magnetic flux density, metaphase, micronuclei, mutation, nuclear division index, proliferation index, RNA, significant, sister chromatid exchange, synergistic, X-rays

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