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Medical/biological Study (experimental study)Magnetic-field-induced DNA strand breaks in brain cells of the rat. med./biol. By: Lai H, Singh NP
Published in: Environ Health Perspect 2004; 112 (6): 687 - 694 (  PubMed Entry , Journal web site )Aim of study (according to author)
To investigate the effects of a 60 Hz magnetic field exposure on brain cell DNA in the rat.
Background/further details:
Male rats (8/group) were exposed and additionally treated with the free radical scavenger Trolox (a vitamin E analog), the nitric oxide synthase inhibitor 7-nitroindazole and the iron chelator deferiprone to investigate their role on magnetic field induced DNA damage.
In another treatment group the effects of an acute (2 hours at 0.5 mT) magnetic field exposure on apoptosis and necrosis in rat brain cells were examined. Endpoint Exposure
General category: magnetic field, 50/60 Hz (AC)
FIELD View further expo parametersExposed system:
animal (species/strain): rat/Sprague-Dawley
whole body exposure Methods
Endpoint/Measurement parameters/Methodology investigated material: DNA/RNA (in vitro), intact cell/cell culture (in vitro)
investigated organ system: brain/CNS
time of investigation: after exposure
Main outcome of study (according to author)
The exposure to a 60 Hz magnetic field caused a significant increase in DNA single-strand breaks and in DNA double-strand breaks. Prolonging the exposure to 48 hours caused a larger increase.
The acute magnetic field exposure also increased apoptosis and necrosis of brain cells in the rat.
The treatment with Trolox or 7-nitroindazole blocked the magnetic field-induced DNA strand breaks. These data further support a role of free radicals on the magnetic field induced DNA damage (cf. publication 1268).
The treatment with the iron chelator also blocked the effects of magnetic fields on brain cell DNA.
These findings suggest that the exposure to a 60 Hz magnetic field initiates an iron-mediated process (e.g., the Fenton reaction) that increases free radical formation in brain cells, leading to DNA strand breaks and cell death. (Study character: medical/biological study, experimental study, full/main study, blind study)
Study funded by - National Institute of Environmental Health Sciences (NIEHS), North Carolina, USA
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Glossary: 60 Hz, AC, acute, analog, animal, apoptosis, biological, blind study, brain, cell, cell culture, cell death, cell division, cell viability, chelator, CNS, DNA, DNA strand breaks, double-strand breaks, endpoint, exposed, exposure, Fenton reaction, fluorescence microscopy, free radical, free radical scavenger, full/main study, genotoxicity, induced, inhibitor, in vitro, iron, magnetic field, magnetic flux density, microgel electrophoresis assay, migration, mutation, necrosis, nitric oxide synthase, proliferation, rat, rat/Sprague-Dawley, RNA, significant, single-strand breaks, species, strain, vitamin E, whole body exposure |
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