• experimental
  
• epidemiological
  

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Medical/biological Study (experimental study)

Cell type-specific genotoxic effects of intermittent extremely low-frequency electromagnetic fields. med./biol.

PubMed Entry

Journal web site

Aim of study (according to author)
To study whether intermittent extremely low frequency electromagnetic fields exposure causes similar effects in cell types other than human fibroblasts.
Background/further details:
Temperature was continuously monitored and temperature difference between the chambers did not exceed 0.3°C.

Endpoint

• genotoxicity/mutation: DNA damage

Exposure
General category: magnetic field, low frequency field, 50/60 Hz (AC)

Field characteristics	Parameters
50 Hz exposure duration: intermittent, 5 min on/10 min off, for 1 to 24 h in 1 h steps	magnetic flux density: 1 mT

Exposed system:
intact cell/cell culture (in vitro)
human diploid fibroblast cells; SV40-transformed GFSH-R17 rat granulosa cells; human melanocytes; skeletal muscle cells; human monocytes; human lymphocytes

Methods
Endpoint/Measurement parameters/Methodology

• genotoxicity/mutation: DNA damage, DNA strand breaks (alkaline and neutral comet assay)

Main outcome of study (according to author)
The data show that the induction of DNA strand breaks by exposure to intermittent extremely low frequency electromagnetic fields is cell type-specific. The authors could identify three responder (human fibroblasts, human melanocytes, rat granulosa cells) and three non-responder cell types (human lymphocytes, human monocytes, human skeletal muscle cells), which points to the significance of the cell system used when investigating genotoxic effects.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

• European Union (EU)/European Commission

• by  Rudiger HW, Kratochvil E, Pilger A

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