• experimental
  
• epidemiological
  

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Medical/biological Study (experimental study)

Effect of high-density extremely low frequency magnetic field on sister chromatid exchanges in mouse m5S cells. med./biol.

PubMed Entry

Journal web site

Aim of study (according to author)
To investigate the effects on the DNA and the cell survival of mouse cells exposed to extremely low frequency magnetic field in vitro.
Background/further details:
The cells were exposed to extremely low frequency magnetic field alone, treated with mitomycin C (4, 10 and 40 nM) alone or treated with "mitomycin C plus extremely low frequency magnetic field exposure".

Endpoint

• genotoxicity/mutation: DNA damage

Exposure
General category: magnetic field, 50/60 Hz (AC)

Field characteristics	Parameters
field 1: 60 Hz exposure duration: continuous for 42 h	magnetic flux density: 50 mT effective value
field 2: 60 Hz exposure duration: continuous for 42 h	magnetic flux density: 5 mT effective value
field 3: 60 Hz exposure duration: continuous for 42 h	magnetic flux density: 400 mT effective value

Exposed system:
intact cell/cell culture (in vitro)
mouse m5S cells

Methods
Endpoint/Measurement parameters/Methodology

• genotoxicity/mutation: sister chromatid exchange, number of sister chromatid exchanges/chromosome (fluorescence plus Giemsa staining)
• cell viability/cell division/proliferation: cell survival (number of survival colonies), cell cycle progression (proliferation index)

Main outcome of study (according to author)
The cells exposed to 400 mT extremely low frequency magnetic field exhibited a significant elevation of the sister chromatid exchange frequencies. No significant difference was observed in cells exposed to 5 and 50 mT extremely low frequency magnetic field.
The exposure to extremely low frequency magnetic field did not influence the proliferation index value.The proliferation index value in mitomycin C treated cells and in "mitomycin C treated cells plus extremely low frequency magnetic field exposure" were significantly lower.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

• Ministry of Education, Culture, Sports, Science and Technology, Japan

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