• experimental
  
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Medical/biological Study (experimental study)

50-Hz, 1-mT sinusoidal magnetic fields do not affect micronucleus frequency and cell proliferation in human lymphocytes from normal and Turner's syndrome subjects. med./biol.

Journal web site

Aim of study (according to author)
To investigate the effect of a sinusoidal magnetic field (50 Hz, 1 mT) on cell proliferation and micronucleus frequency in human lymphocytes in vitro.
Background/further details:
A previous study to pulsed magnetic field exposure (publication 2071) was performed,- here a sinusoidal magnetic field exposure was applied.
Lymphocyte cultures from five subjects affected by Turner's syndrome (mean age 14 years) and from five healthy donors (mean age 28 years) were examined. From each sample a electromagnetic field exposed and sham exposed culture was set up.

Endpoints

• genotoxicity/mutation: micronucleus formation
• cell viability/cell division/proliferation: cell proliferation

Exposure
General category: magnetic field, 50/60 Hz (AC)

Field characteristics	Parameters
50 Hz exposure duration: 72 h	magnetic flux density: 1 mT effective value

Exposed system:
intact cell/cell culture (in vitro)

Methods
Endpoint/Measurement parameters/Methodology

• genotoxicity/mutation: micronucleus formation (cytokinesis block assay)
• cell viability/cell division/proliferation: cell proliferation (mean number of cell cycles per cell, cytokinesis block proliferation index)

Main outcome of study (according to author)
No statistically significant differences in micronucleus frequency and cell proliferation were observed between control group and exposed group from healthy and Turner`s syndrome subjects.
These data indicate that the electromagnetic field investigated does not exert any genotoxic effect nor interfere with cell cycle.

(Study character: medical/biological study, experimental study, full/main study, blind study)

Study funded by

• National Research Council (CNR; Consiglio Nazionale delle Ricerche); Italy

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