Exposure details   Elevated sister chromatid exchange frequencies in dividing human peripheral blood lymphocytes exposed to 50 Hz magnetic fields. by Wahab MA, Podd JV, Rapley BI, Rowland RE published in: Bioelectromagnetics 2007; 28 (4): 281 - 288 Exposure 1: Main characteristics frequency: 50 Hz type of field: magnetic field signal form: sinusoidal Exposure setup exposure source/structure: Helmholtz coils exposure time pattern: continuous for 72 h chamber details: The Helmholtz coils were housed in a purpose-built incubator situated in an electrically shielded room away from any MF sources and maintained at 37 °C by a flow of warm air from a distant source (2 m). setup details: Culture tubes containing 5 ml of medium were placed within the pair of Helmholtz coils. Each coil was 180 mm in diameter and made of 100 turns of enamelled 0.7 mm diameter copper wire. sham: sham exposure was performed add. information: Control tubes were similarly cultured over a separate 72 h incubation period, but received no exposure to MFs. An independent person set the MF parameters that were unknown to the researcher. Exposure parameters parameter: value: method: remark: magnetic flux density  1 µT  calculated  -  magnetic flux density  1 mT  calculated  -  measurement and calculation details: To determine the exact nature of the field produced by the coil, the system was modelled using the EMF simulation program, MagneSim, developed by Foster and Page [Rapley et al., 1998]. Exposure 2: Main characteristics frequency: 50 Hz type of field: magnetic field signal form: sinusoidal Exposure setup exposure source/structure: same setup as in Exposure 1 exposure time pattern: intermittent, 4 s on/4 s off, for 72 h Exposure parameters parameter: value: method: remark: magnetic flux density  1 µT  calculated  -  magnetic flux density  1 mT  calculated  -  Exposure 3: Main characteristics frequency: 50 Hz type of field: magnetic field signal form: rectangular Exposure setup exposure source/structure: same setup as in Exposure 1 exposure time pattern: continuous for 72 h Exposure parameters parameter: value: method: remark: magnetic flux density  1 µT  calculated  -  magnetic flux density  1 mT  calculated  -  Exposure 4: Main characteristics frequency: 50 Hz type of field: magnetic field signal form: rectangular Exposure setup exposure source/structure: same setup as in Exposure 1 exposure time pattern: intermittent, 4 s on/4 s off, for 72 h Exposure parameters parameter: value: method: remark: magnetic flux density  1 µT  calculated  -  magnetic flux density  1 mT  calculated  -  Reference article Rapley BI et al. (1998): Influence of extremely low frequency magnetic fields on chromosomes and the... Return to general data Glossary: 50 Hz, coil, copper, culture, EMF, exposure, Helmholtz coils, incubation, incubator, intermittent, magnetic flux density, medium, MF, sham exposure, signal, sinusoidal

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