The exposure system consisted of four WPCs, each fed by a different power level in a blinded procedure. Two WPCs shielded by metal grid boxes were placed in each of two incubators (5% CO2 and 95% air). Each WPC or at least each incubator had a dummy culture Petri dish for temperature measurement.
The WPC [Laval et al., 2000] consisted of two parallel plates (15 x 15 x 2.9 cm³), short-circuited at the edges by four posts. The outer conductor of the RF supply coaxial cable was connected to the top plate, and the inner conductor was connected to the ground plane. The temperature of the exposed samples was maintained at 36.9 ± 0.5°C by spiral coils with circulating water positioned on each plate. Four 35-mm Petri dishes containing 3 ml of diluted blood were placed inside the cell where the E-field distribution was quite uniform.
Six conditions were tested in duplicate: RF exposure at peak SARs of 0 (sham), 1, 5 and 10 W/kg, a positive (MMC-treated) control, and a negative (unexposed and untreated) control. Positive and negative controls were kept for 24 h in a third CO2 incubator at 37°C.
The SAR distribution was determined by numerical and experimental dosimetry. In the numerical model using the Finite Integration Technique (FIT), the Petri dishes were filled with 1 ml of blood at the bottom and 2 ml of culture medium on top. The simulations showed that almost 90% of the blood volume was exposed at about 0.30 W/kg per W, and the measured efficiency was about 0.35 ± 0.10 W/kg per W.
Die Ergebnisse zeigten keine genotoxischen oder zytotoxischen Wirkungen im Bereich der untersuchten SAR-Werte. Die Ergebnisse wurden durch zwei Forschergruppen und bei Bewertung derselben Objektträger durch zwei Mitarbeiter bestätigt.
Zeni O et al.
Induction of an adaptive response in human blood lymphocytes exposed to radiofrequency fields: Influence of the universal mobile telecommunication system (UMTS) signal and the specific absorption rate.
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