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Medizinische/biologische Studie (experimentelle Studie)

Comparative study of cell cycle kinetics and induction of apoptosis or necrosis after exposure of human mono mac 6 cells to radiofrequency radiation.

[Vergleichsstudie zur Kinetik des Zellzyklus und der Apoptose- oder Nekrose-Induktion nach Hochfrequenz-Befeldung menschlicher Mono Mac 6 Zellen].

Veröffentlicht in: Radiat Res 2006; 166 (3): 539-543

Ziel der Studie (lt. Autor)

Es sollte der Einfluss hochfrequenter elektromagnetischer Felder in vitro auf den Zellzyklus, die BrdU-Aufnahme und die Induktion der Apoptose und Nekrose menschlicher Mono Mac 6 Zellen untersucht werden.
Hintergrund/weitere Details: Als Positivkontrolle dienten Zellen, die mit Gliotoxin oder Tetradecanoylphorbolacetat (PMA) behandelt wurden.

Endpunkt

Exposition/Befeldung (teilweise nur auf Englisch)

Exposition Parameter
Exposition 1: 1.800 MHz
Modulationsart: gepulst
Expositionsdauer: kontinuierlich für 12 h
  • SAR: 2 W/kg nicht spezifiziert
Exposition 1
Hauptcharakteristika
Frequenz 1.800 MHz
Typ
Charakteristik
  • gerichtetes Feld
Expositionsdauer kontinuierlich für 12 h
Modulation
Modulationsart gepulst
Zusatzinfo GSM-DTX mode, with transmission reduced to 12 active frames per 104 frames, leading to a crest factor of 69. The frame structure results in 2, 8, and 217 Hz components. [Lantow et al., 2006]
Expositionsaufbau
Expositionsquelle
Kammer Two single-mode resonator cavities were placed inside a CO2 incubator that were randomly and blindly assigned for exposure [Schuderer et al., 2004].
Aufbau Six 35-mm Petri dishes were placed in each waveguide on a dish holder ensuring the correct placement in the H-field maxima of the standing waves.
Scheinexposition Eine Scheinexposition wurde durchgeführt.
Zusatzinfo Cells were treated simultaneously in the same incubator under the following conditions: untreated (sham or incubator control), RF exposed, sham exposed + PMA, RF exposed + PMA, sham exposed + gliotoxin, RF exposed + gliotoxin.
Parameter
Messgröße Wert Typ Methode Masse Bemerkungen
SAR 2 W/kg nicht spezifiziert nicht spezifiziert - -
Referenzartikel
  • Lantow M et al. (2006): Free radical release and HSP70 expression in two human immune-relevant cell lines after exposure to 1800 MHz radiofrequency radiation.
  • Schuderer J et al. (2004): High Peak SAR Exposure Unit With Tight Exposure and Environmental Control for In Vitro Experiments at 1800 MHz
Exponiertes System:

Methoden Endpunkt/Messparameter/Methodik

Untersuchtes Material:
Untersuchungszeitpunkt:
  • vor der Befeldung
  • nach der Befeldung

Hauptergebnis der Studie (lt. Autor)

Es wurden keine statistisch signifikanten Unterschiede in der Apoptose- oder Nekrose-Induktion, in der Zellzyklus-Kinetik oder in der Aufnahme von BrdU nach hochfrequenter elektromagnetischer Befeldung festgestellt. Ein signifikanter Anstieg an apoptotischen und nekrotischen Zellen wurde in den mit Gliotoxin- und PMA- behandelten Zellen der Positivkontrolle beobachtet.
Es zeigten sich in der Zellzyklus-Analyse oder BrdU-Aufnahme während 72 Stunden nach Exposition keine Unterschiede zwischen den hochfrequent befeldeten oder schein-exponierten Zellen, wohingegen die Behandlung mit PMA eine signifikante Akkumulierung von Zellen in der G1-Phase und eine Verminderung der Zellen in der S-Phase induzierte.
Studienmerkmale:

Studie gefördert durch

  • Bundesamt für Strahlenschutz (BfS; Federal Office for Radiation Protection), Salzgitter, Germany
  • Deutsches Mobilfunk Forschungsprogramm (DMF; German Mobile Phone Research Programme) at Federal Office for Radiation Protection (BfS)

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  • Palumbo R et al. (2008): Exposure to 900 MHz radiofrequency radiation induces caspase 3 activation in proliferating human lymphocytes.
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  • Maes A et al. (2006): Cytogenetic investigation of subjects professionally exposed to radiofrequency radiation.
  • Joubert V et al. (2006): Microwave exposure of neuronal cells in vitro: Study of apoptosis.
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  • Scarfi MR et al. (2006): Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes: an interlaboratory study.
  • Gurisik E et al. (2006): An in vitro study of the effects of exposure to a GSM signal in two human cell lines: monocytic U937 and neuroblastoma SK-N-SH.
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