Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells med./bio.

[Wiederholte Exposition bei einem zeitveränderlichen 60 Hz-Magnetfeld induziert DNA-Doppelstrangbrüche und Apoptose in menschlichen Zellen]

Ziel der Studie (lt. Autor)

Endpunkt

• Genotoxizität/Mutation: DNA-Schaden
• Zelllebensfähigkeit/Zellteilung/Zellproliferation: Apoptose

Exposition/Befeldung (teilweise nur auf Englisch)

• • 50/60 Hz
  • magnetisches Feld

Vollständige Ansicht Kompaktansicht

Methoden Endpunkt/Messparameter/Methodik

• Genotoxizität/Mutation: DNA-Schaden (Doppelstrangbruch; Bildung von gamma-H2AX-Foci (Fluoreszenzmikroskopie) und Phosphorylierung von gamma-H2AX (Western BLot)) und DNA-Schaden-Kontrollpunkt-Signalweg (phospho-Checkpoint-Kinase 2-Expression; Western Blot)
• molekulare Biosynthese: Expression der Stress-aktivierten Proteinkinasen ((phospho-) P38-Mitogen aktivierte Proteinkinase und (phospho-) JNK Mitogen aktivierte Proteinkinase; Western Blot)
• Zelllebensfähigkeit/Zellteilung/Zellproliferation: Apoptose und Apoptose-verbundene Signalwege (Expression von Caspase-3, aktivierter Caspase-3 und PARP; Western Blot), Zellproliferation/Zelllebensfähigkeit (MTT-Test)

Hauptergebnis der Studie (lt. Autor)

Studie gefördert durch

• Korea Research Institute of Chemical Technology (KRICT)
• Ministry of Knowledge Economy (MKE), Korea
• Yonsei University Research Fund, Korea

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