Frisch geschlüpfte Insekten wurden auf 12 identische Gruppen von je 10 Männchen und 10 Weibchen aufgeteilt und kontinuierlich während der ersten 5 Tage ihres erwachsenen Lebens bei 3 verschiedenen Stärken exponiert oder schein-exponiert. Die ersten 48 Stunden wurden die Männchen und Weibchen in verschiedenen Glasröhrchen gehalten. Nach diesen 48 Stunden, wenn Weibchen und Männchen voll geschlechtsreif waren, wurden sie für 72 Stunden in einem anderen Glasröhrchen zusammengesetzt, wo sie sich paaren und Eier legen konnten. Nach diesen 5 Tagen wurden die Fliegen aus dem Röhrchen entfernt. Die Weibchen wurden seziert und die Ovarien auf DNA-Schäden hin untersucht. Die Röhrchen mit den sich entwickelnden Embryonen wurden für weitere 6 Tage in einem Kultivierungsraum ohne Expositioninkubiert.
glass tubes with flys were put in the centre of the air cores of the coils, suspended by nylon strings
Aufbau
two nearly identical cylindrical coils were used; both coils had a length of 0.25 m, a radius of 7.5 x 10-2 m and a diameter of insulated wire of 2 x 10-3 m; number of turns in each coil = 330; in the first coil, the turns were parallel and in the same direction, thus generating a magnetic field; in the second coil, half of the turns were antiparallel (parallel but in opposite direction), so that the magnetic field (and the magnetically induced electric field) in a region of about 12 cm width around the centre of the coil was zero (used for sham exposure)
after it was checked by preliminary experiments that the sequence (mutual position) of the two coils did not affect the outcome of the experiments, their positions were always the same during all experiments, at a certain place of the laboratory with the minimum stray 50-Hz fields and in at least 2 m distance between each other so that the fields of the "exposure coil" did not affect the "sham exposure coil"
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