To test the hypothesis that the 930 MHz continuous wave (CW) electromagnetic field, which is the carrier of signals emitted by mobile phones, affects the reactive oxygen species (ROS) level in living cells.
|Exposure duration||continuous for 5 or 15 min|
|Setup||Plastic tubes (15 mm diameter) containing lymphocyte suspensions treated with 10 µg/ml ferrous chloride or distilled H2O respectively were exposed in a tightly closed Plexiglas container (26 cm long, 17 cm diameter) which was placed in the GTEM cell. An identical container with unexposed samples was placed outside the GTEM cell (in the natural electromagnetic field).|
|Additional info||The experiment included four groups of lymphocytes: group I, not treated with ferrous ions and unexposed (control group); group II, not treated with ferrous ions and exposed; group III, treated with ferrous ions and unexposed; and group IV, treated with ferrous ions and exposed to electromagnetic radiation. Experiments were repeated twice, with 1 week interval between each replicate using rats from these same litter.|
The data show that acute exposure does not affect the number of produced ROS. If, however, iron ions (FeCl2) with final concentration 10 µg/ml) was added to the lymphocyte suspensions to stimulate ROS production, after both durations of exposure (5 and 15 min), the magnitude of fluorescence (ROS level during the experiment) was significantly greater in the irradiated cells.