All of the transcripts investigated are wound-up-regulated. Thus, similarities between wounding and radiofrequency electromagnetic field exposure could be studied. Additionally, since two of them encode calcium-binding proteins, the authors examined the effects of varying calcium availability to determine if there was any feedback mechanism involved in their transcription.
ATP concentration and adenylate energy charge were also measured after exposure, as well as the effect of an uncoupling agent on transcript accumulation in order to assess the influence of radiofrequency exposure on cellular energy status and to correlate this with changes in mRNA abundances.
Exposure duration: continuous for 10 min
|Exposure duration||continuous for 10 min|
|Chamber||Seeds were grown inside two identical custom-made (plywood) culture chambers with a hydroponic system. One of the chambers was armoured with a multi-layered aluminum material to protect the plants from exposure.|
|Setup||When the plants were 3 weeks old, the whole culture chamber was placed in the MSRC (24 h prior to irradiation) in a volume where the field was homogeneous and isotropic. The MSRC (mode stirred reverberation chamber) was a large (200 m³) Faraday cage with an RF-EMF that irradiated plants from several directions with different polarizations (due to reflection properties of the MSRC).|
|electric field strength||5 V/m||-||-||-||-|
The data provide new evidence supporting the hypothesis that plants perceive and respond to microwave exposure as though it was an injurious treatment. In addition to the rapidity of the response (stress-related mRNA accumulation) and its dependency on the second messenger calcium, the authors observed a strong correlation between all the parameters measured (total and polysomal transcript abundance, ATP concentration and adenylate energy charge.