Study type: Medical/biological study (experimental study)

Reactive oxygen species formation is not enhanced by exposure to UMTS 1950 MHz radiation and co-exposure to ferrous ions in Jurkat cells. med./bio.

Published in: Bioelectromagnetics 2009; 30 (7): 525-535

Aim of study (acc. to author)

To study cell viability and reactive oxygen species formation in human lymphoblastoid cells exposed to 1950 MHz, UMTS signal, for short (1 h) or long (24 h) exposure duration. To investigate if radiofrequency exposure may modify reactive oxygen species production induced by a well known oxidative stress inducer, co-exposures with ferrous ions (Fe2+, administered as iron sulfate FeSO4) were also carried out (concurrent or after radiofrequency exposure).

Background/further details

Ferrous ions act by producing a very reactive free radical, the hydroxyl radical (OH.). Positive controls were treated with iron sulfate for the same duration.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,950 MHz
Exposure duration: 5 min - 60 min or 24 h

General information

experiments were conducted under 4 different conditions: i) 5 min - 60 min RF only ii) 24 h RF only iii) 5 min - 60 min RF + FeSO4 iv) 24 h RF + FeSO4

Exposure 1

Main characteristics
Frequency 1,950 MHz
Type
Exposure duration 5 min - 60 min or 24 h
Exposure setup
Exposure source
Setup rectangular short-circuited waveguide (109.2 mm x 54.6 mm x 500 mm) in TE10 mode; cell cultures placed on a plastic stand inside the waveguide; electric field parallel to the sample surface
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.5 W/kg - calculated - -
SAR 2 W/kg - calculated - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data indicate that non-thermal radiofrequency exposures do not increase spontaneous reactive oxygen species formation in any of the experimental conditions investigated. Consistent with the lack of reactive oxygen species formation, no change in cell viability was found in radiofrequency exposed cells.
Similar results were obtained when co-exposures were considered: combined exposures to radiofrequency and iron sulfate (as stress-inducer) did not increase reactive oxygen species production induced by the chemical treatment alone.

Study character:

Study funded by

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