Medical/biological study (experimental study)

Effects of extremely low frequency magnetic field on oxidative balance in brain of rats med./bio.

By: Ciejka E, Kleniewska P, Skibska B, Goraca A

Published in: J Physiol Pharmacol 2011; 62 (6): 657-661

Aim of study (acc. to author)

To study the effect of extremely low frequency magnetic fields most frequently used in magnetotherapy on reactive oxygen species generation in brain tissue of rats depending on the time of exposure.

Background/further details

30 animals were divided into one sham exposure group (n=10) and two exposure groups (each n=10; two different exposure durations: 30 min/day and 60 min/day).

Endpoint

effects on the neurological system: oxidative stress in the brain

Exposure

- 40 Hz
- magnetic field
- low frequency
- therapeutic/medical device

Exposure	Parameters
Exposure 1: 40 Hz Exposure duration: 30 min/day (group ii) respectively 60 min/day (group iii) for 10 days between 7 a.m. and 10 a.m.	magnetic flux density: 7 mT

General information

30 rats were divided into three groups of 10 animals each: i) control group ii) and iii) experimental groups with different exposure duration

Exposure 1

Main characteristics

Frequency	40 Hz
Type	magnetic field
Waveform	rectangular
Exposure duration	30 min/day (group ii) respectively 60 min/day (group iii) for 10 days between 7 a.m. and 10 a.m.

Exposure setup

Exposure source	apparatus used in magnetotherapy (MAGNETRONIK MF-10)
Setup	rats in plastic containers were placed inside an applicator being part of the set for magnetotherapy
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	7 mT	-	-	-	-

Exposed system:

Methods Endpoint/measurement parameters/methodology

effects on the neurological system: oxidative stress in the brain (lipid peroxidation (via thiobarbituric acid reactive substances and spectrophotometry); protein content (spectrophotometry), level of free sulfhydryl groups (spectrophotometry); level of hydrogen peroxide (spectrofluorometry)

Investigated system:

brain homogenates and supernatants

Investigated organ system:

brain/CNS

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Extremely low frequency magnetic fields of 7 mT, 40 Hz, 30 min/day for 10 days caused a significant increase in lipid peroxidation and insignificant increases in hydrogen peroxide and free sulfhydryl groups as well as an insignificant decrease in the total protein content (as compared with the control group).
The same extremely low frequency magnetic fields but applied for 60 min/day caused insignificant increases in lipid peroxidation and hydrogen peroxide as well as significant increases in free sulfhydryl groups and protein concentration in the brain homogenates (as compared with the control group).
The effect of extremely low frequency magnetic field irradiation on oxidative stress parameters depended on the duration of magnetic field exposure.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Medical University of Lodz, Poland

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