Study type: Medical/biological study (experimental study)

Fractal analysis of extra-embryonic vascularization in Japanese quail embryos exposed to extremely low frequency magnetic fields. med./bio.

Published in: Bioelectromagnetics 2013; 34 (2): 114-121

Aim of study (acc. to author)

To examine the effect of an exposure to extremely low frequency magnetic fields on the development of blood vessels of the yolk sac membrane in quail embryos.

Background/further details

The eggs were incubated (37.5°C) for 96 hours. On the second day of incubation, a small window was cut into the egg shell and covered with a special foil, hence the vascular network could be photographed. Exposure started after an incubation period of 48 hours. Observations were performed after 72 and 96 hours.
6 groups of eggs were examined: 1.) control group (n=7), 2.) 3 x 1 h exposure daily with a 7 h interval between exposures (n=6), 3.) 3 x 2 h exposure daily with a 6 h interval between exposures (n=7), 4.) 3 x 3 h exposure daily with a 5 h interval between exposures (n=14), 5.) 3 x 4 h daily with a 4 h interval between exposures (n=10) and 6.) continuous for 24 h daily (n=15).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: intermittent for 3, 6, 9, 12 or 24 h/day for 2 days (see add. information)

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration intermittent for 3, 6, 9, 12 or 24 h/day for 2 days (see add. information)
Exposure setup
Exposure source
Setup pair of Helmholtz coils with a diameter of 38 cm and 154 turns of wire each; connected in parallel; eggs placed in an incubator along the horizontal axis between the coils
Additional info exposure time: group I: 3 x 1 h/day with a 7 h interval between exposures group II: 3 x 2 h/day with a 6 h interval between exposures group III: 3 x 3 h/day with a 5 h interval between exposures group IV: 3 x 4 h/day with a 4 h interval between exposures group V: continuous for 24 h/day
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.13 mT effective value measured - in the coil's center
magnetic flux density 0.9 mT effective value measured - at the coil's edge

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

In the groups exposed for 3 x 2 h/day and 3 x 3 h/day (groups 3 and 4), the development of the vascular network was inhibited after 72 hours of incubation compared to the control group. No differences between the groups were detected after an incubation time of 96 hours.
The authors conclude that exposure to an extremely low frequency magnetic field could influence the development of blood vessels in a window effect.

Study character:

Study funded by

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