Study type: Medical/biological study (experimental study)

Age-Dependent Effects of ELF-MF on Oxidative Stress in the Brain of Mongolian Gerbils. med./bio.

Published in: Cell Biochem Biophys 2013; 66 (3): 513-521

Aim of study (acc. to author)

To study the effects of extremely low frequency magnetic fields on oxidative stress in the brain of adult (3 months old) and middle-aged (10 month old) gerbils.

Background/further details

Groups of four gerbils per cage were used: cage control, sham exposed group and three different exposure groups. The gerbils from sham exposed and exposed groups were subdivided into two groups, one sacrificed on the 7th day (immediately after the exposure) and the other one sacrificed on the 10th day from the beginning of the experiment.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 7 days

General information

Animals were exposed at three different magnetic flux densities.

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 7 days
Exposure setup
Exposure source
  • elctromagnet
Setup electromagnet with a regular laminated transformer core and a pole diameter of 9.5 cm; gerbils placed in 26 cm x 43 cm x 15 cm polycarbonate cages; two cages with 3-4 gerbils positioned simultaneously near the magnet, one on the left and the other on the right side with a distance between 20 cm and 40 cm
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 8.9 mT maximum measured - on the electromagnetic poles
magnetic flux density 0.5 mT average over time measured - in the cages
magnetic flux density 0.25 mT average over time measured - in the cages
magnetic flux density 0.1 mT average over time measured - in the cages

Reference articles

  • Janac B et al. (2012): Temporal patterns of extremely low frequency magnetic field-induced motor behavior changes in Mongolian gerbils of different age.
  • Nikolic LM et al. (2010): Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In all gerbils, extremely low frequency magnetic field exposure significantly increased oxidative stress in all investigated brain regions. This effect increased in a magnetic flux density intensity dependent manner and was higher in middle-aged gerbils. Three days after exposure termination, the values of oxidative stress parameters showed a tendency to return to their control levels, particularly in 3-month-old gerbils.
These findings indicate that an extremely low frequency magnetic field exposure is able to induce oxidative stress in the brain of gerbils in an age- and magnetic field intensity-dependent manner.

Study character:

Study funded by

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