To measure the distribution of erythrocytes and blood plasma in various organs of mice. For these purposes erythrocytes were double radioactive labelled extracorporeal (outside the body) and than reinjected into mice. Letal microwave exposure was used to prevent blood flow during organ isolation by coagulation. The results were compared with those obtained by sacrificing animals by decapitation.
Exposure duration: depending on weight of mice
The loss of blood caused by decapitation compared to sacrifice with microwave was in the range of 6% (for kidney) to more than 50% (for brain). Therefore the double isotope/microwave method is a valuable technique to determine plasma and erythrocyte values (and within hematocrit).