Study type: Medical/biological study (experimental study)

Hyperthermia inhibits angiogenesis med./bio.

By: Fajardo LF, Prionas SD, Kowalski J, Kwan HH
Published in: Radiat Res 1988; 114 (2): 297-306
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Aim of study (acc. to author)

To determine the in vivo heat sensitivity of blood capillaries and their endothelial cells.

Background/further details

Angiogenesis discs (a sponge model) were implanted subcutaneously in mice. Vascular growth was stimulated by slow release of epidermal growth factor placed in the center of each disc. After 5 days of growth the discs were subjected to radiofrequency-induced hyperthermia (the thermal doses utilized were 41, 42, 43, and 44°C).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 500 kHz
Exposure duration: 30 min
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Exposure 1

Main characteristics
Frequency 500 kHz
Type
Exposure duration 30 min
Exposure setup
Exposure source
Setup Animals placed with the left side lying flat on a metal platform while the upper electrode 12 mm in diameter tightly placed against the shaved skin of the animal.
Parameters

No parameters are specified for this exposure.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

An inverse relationship was revealed between vessel growth and exposure temperature. The extent of the fibroblastic growth was also inversely proportional to temperature.
Thus, in this system, the microvasculature shows dose-dependent damage by hyperthermia. This inhibition of angiogenesis may result from endothelial cell killing, interference with cell replication, inhibition of cell migration, or a combination of these mechanisms.

Study character:

Study funded by