Study type: Medical/biological study (experimental study)

Electric field-directed growth and branching of cultured frog nerves: effects of aminoglycosides and polycations. med./bio.

Published in: J Neurobiol 1995; 26 (4): 523-536

Aim of study (acc. to author)

To investigate the growth rate, branching behavior, and orientation of cultured embryonic frog nerves exposed to different stimuli. Cells were treated to DC electric fields, to raised extracellular cations, aminoglycoside antibiotics, or to combinations of these stimuli.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuous for 5 h

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuous for 5 h
Exposure setup
Exposure source
Chamber culture chamber/64 mm x 10 mm x 0.5 mm
Setup Ag/AgCl electrodes electrodes connected though 15 cm Agar-salt bridge
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 500 mV/cm minimum - - -
electric field strength 2,250 mV/cm maximum - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure

Main outcome of study (acc. to author)

Electric field exposure leads to cathodal orientation and cathodal branching of the neurites, which could be blocked by aminoglycosid antibiotica treatment (Neomycin, Gentamicin, and Tobramycin were used) and by raised extracellular amounts of divalent cations (magnesium and calcium were used) respectively. However, neomycin in combination with high external calcium levels leads to an anodal orientation and branching of the neurites. Aminoglycoside treatment decreased neurite growth rate. In the case of neomycin this effect was reversed by calcium. Growth rate in a field-strength dependet manner was altered by raised calcium but not by magnesium.

Study character:

Study funded by