Study type: Medical/biological study (experimental study)

Effect of 7 mT static magnetic field and iron ions on rat lymphocytes: apoptosis, necrosis and free radical processes. med./bio.

Published in: Bioelectrochemistry 2002; 57 (2): 107-111

Aim of study (acc. to author)

To study whether the oxidative DNA damage caused by simultaneous exposure of rat lymphocytes to a 7 mT static magnetic field and iron ions may lead to cell death: apoptosis or necrosis.

Background/further details

In previous studies the authors have shown that simultaneous exposure of rat lymphocytes to 7 mT magnetic field and iron ions caused a significant increase in the number of cells with DNA damage, and that melatonin, an established free radical scavenger, provides protection against that DNA damage (see publication 4790 and publication 8598).
During static magnetic field exposure, some samples were treated with ferrous chloride (FeCl2, 10 µg/ml), the rest served as controls.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuous for 3 h

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuous for 3 h
Exposure setup
Exposure source
Setup Lymphocyte suspension was exposed in a small water bath, which was placed inside the coils. The control samples were kept outside the coils.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 7 mT - measured unspecified -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant differences were found between unexposed lymphocytes and lymphocytes exposed to the static magnetic field. Incubation with FeCl2 did not affect cell viability.
However, when cells were exposed to the static magnetic field and simultaneously treated with FeCl2, there was a significant increase in the percentage of apoptotic and necrotic cells accompanied by significant alterations in cell viability.
As compared to lipid peroxidation, there is a significant increase in the amount of lipid peroxidation end products malondialdehyde + 4-hydroxynonenal in lymphocytes after simultaneous exposure to the static magnetic field and FeCl2 compared to the control samples and those exposed to the magnetic field alone. This indicates that 7 mT static magnetic field in the presence of ferrous ions can increase the concentration of oxygen free radicals and thus may lead to cell death.

Study character:

Study funded by

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