研究のタイプ: 医学/生物学の研究 (experimental study)

[1.9 GHzのパルス変調無線周波にインビトロで暴露されたヒト神経膠芽腫細胞株のマイクロアレイ遺伝子発現解析] med./bio.

Microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field.

掲載誌: Radiat Res 2006; 165 (6): 636-644

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研究目的(著者による)

To assess the ability of exposure to a 1.9 GHz pulse modulated radiofrequency field to affect gene expression in glioblastoma cells.

詳細情報

Positive controls were performed at 43°C (1 h).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1.9 GHz
Modulation type: pulsed
ばく露時間: continuous for 4 h
  • SAR: 0.1 W/kg mean (±24%)
  • SAR: 1 W/kg mean (±24%)
  • SAR: 10 W/kg mean (±24%)

ばく露1

主たる特性
周波数 1.9 GHz
タイプ
  • electromagnetic field
特性
  • guided field
偏波
  • circular
ばく露時間 continuous for 4 h
Modulation
Modulation type pulsed
Duty cycle 33.3 %
Repetition frequency 50 Hz
ばく露装置
ばく露の発生源/構造
チャンバの詳細 The temperature in the cell cultures was maintained at 37.0 ± 0.5°C by a mixture of prewarmed 95% air and 5% CO2.
ばく露装置の詳細 Cells were exposed in 10 ml of culture medium in 60-mm Petri dishes using a series of six circularly polarized cylindrical waveguides.
Sham exposure A sham exposure was conducted.
Additional information Negative and positive (heat shock) controls were run concurrently with each experiment.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.1 W/kg mean 測定値 - ±24%
SAR 1 W/kg mean 測定値 - ±24%
SAR 10 W/kg mean 測定値 - ±24%

Reference articles

  • Gajda GB et al. (2002): [1.9 GHzのラジオ周波数電磁界に細胞をIn vitroばく露するための円筒状導波管アプリケータ]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data provided no evidence that non-thermal radiofrequency fields can affect gene expression in cultured U87MG cells in comparison to the control groups, whereas exposure to heat shock at 43°C up-regulated a number of typical stress-responsive genes in the positive control group.

研究の種別:

研究助成

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