6人のボランティアからの末梢血液リンパ球をUMTS信号(搬送周波数は1950MHz)に24時間ばく露させた。ばく露は0.5、2.0W/kgのSARで導波管を用いて行い、擬似ばく露サンプルも用意した。アルカリ性コメットアッセーでDNA損傷を調べ、細胞毒性はTrypan Blue法で求めた。結果は、両方のSARレベルで、ばく露群と擬似ばく露で比較して、遺伝子毒性や細胞毒性は見られなかった。1950MHzの24時間in vitroばく露はヒトのリンパ球においてDNA損傷を誘発しない。
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This in vitro study was conducted to investigate possible effects of 1950 MHz continous exposure on human peripherial blood leukocytes.
Peripheral blood samples were taken from six healthy non-smoking males aged beween 27 and 32 years. 2 x 250 randomly selected cells per treatment group were examined. Hydrogen peroxide treated cells were the positive controls.
The specific absorption rates tested were selected to evaluate conditions below (0.5 W/kg) or equal to (2 W/kg) the currently accepted safety limits by the ICNIRP.
For each donor, five conditions were tested in duplicate: RF and sham exposure at two SAR values and positive controls (cultures treated for 30 min with 50-µM hydrogen peroxide). The design of the exposure setup followed the basic requirements suggested in [Kuster et al., 2000].
|ばく露時間||continuous for 24 h|
|Modulation type||cf. additional information|
UMTS signal according to the WCDMA/3GPP standard (five power-controlled user data channels + one control channel)
|ばく露装置の詳細||Four 35-mm Petri dishes filled with 3-ml samples were stacked in a plastic dish holder positioned inside the waveguide|
|Sham exposure||A sham exposure was conducted.|
|Additional information||induced electric field parallel to the sample surface, spatial distribution was quite uniform along the vertical axis; thus, a high uniformity was achieved between the different layers, including the cell layer at the bottom of the Petri dish. As already done in a previous work [Zeni et al., 2005], resonant operation was discarded in order to avoid limitations in the modulation bandwidth of the feeding signal.|
No statistically significant differences were found in continuously exposed and sham exposed human leukocytes concerning cell viability and genotoxicity.