研究のタイプ: 医学／生物学の研究 (experimental study)

[1950 MHz変調信号ばく露後のヒト末梢血リンパ球における細胞毒性と遺伝子毒性の影響評価] med./bio.

Evaluation of Cytotoxic and Genotoxic Effects in Human Peripheral Blood Leukocytes Following Exposure to 1950-MHz Modulated Signal

著者: Sannino A, Calabrese ML, d'Ambrosio G, Massa R, Petraglia G, Mita P, Sarti M, Scarfi MR
掲載誌: IEEE Trans Plasma Sci 2006; 34 (4): 1441-1448
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6人のボランティアからの末梢血液リンパ球をUMTS信号(搬送周波数は1950MHz)に24時間ばく露させた。ばく露は0.5、2.0W/kgのSARで導波管を用いて行い、擬似ばく露サンプルも用意した。アルカリ性コメットアッセーでDNA損傷を調べ、細胞毒性はTrypan Blue法で求めた。結果は、両方のSARレベルで、ばく露群と擬似ばく露で比較して、遺伝子毒性や細胞毒性は見られなかった。1950MHzの24時間in vitroばく露はヒトのリンパ球においてDNA損傷を誘発しない。

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研究目的（著者による）

This in vitro study was conducted to investigate possible effects of 1950 MHz continous exposure on human peripherial blood leukocytes.

詳細情報

Peripheral blood samples were taken from six healthy non-smoking males aged beween 27 and 32 years. 2 x 250 randomly selected cells per treatment group were examined. Hydrogen peroxide treated cells were the positive controls.
The specific absorption rates tested were selected to evaluate conditions below (0.5 W/kg) or equal to (2 W/kg) the currently accepted safety limits by the ICNIRP.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,950 MHz
ばく露時間: continuous for 24 h
  • SAR: 0.5 W/kg average over mass
  • SAR: 2 W/kg average over mass

General information

For each donor, five conditions were tested in duplicate: RF and sham exposure at two SAR values and positive controls (cultures treated for 30 min with 50-µM hydrogen peroxide). The design of the exposure setup followed the basic requirements suggested in [Kuster et al., 2000].

ばく露1

主たる特性
周波数 1,950 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 24 h
Modulation
Modulation type cf. additional information
Additional information

UMTS signal according to the WCDMA/3GPP standard (five power-controlled user data channels + one control channel)
ばく露装置
ばく露の発生源／構造
  • 導波管
  • WR 430: 109.2 mm x 54.6 mm, in TE10 mode
ばく露装置の詳細 Four 35-mm Petri dishes filled with 3-ml samples were stacked in a plastic dish holder positioned inside the waveguide
Sham exposure A sham exposure was conducted.
Additional information induced electric field parallel to the sample surface, spatial distribution was quite uniform along the vertical axis; thus, a high uniformity was achieved between the different layers, including the cell layer at the bottom of the Petri dish. As already done in a previous work [Zeni et al., 2005], resonant operation was discarded in order to avoid limitations in the modulation bandwidth of the feeding signal.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 0.5 W/kg average over mass 測定値および計算値 - -
SAR 2 W/kg average over mass 測定値および計算値 - -
測定および計算の詳細

The numerical and experimental dosimetry carried out is described in [Calabrese et al., 2006]. The efficiency of the system and the SAR distribution inside the samples were evaluated numerically based on the finite integration technique (FIT) as well as experimentally by local temperature measurements carried out in the maximum and minimum local SAR regions [Allis et al., 1977] and by measuring the reflection coefficient of the loaded exposure chamber using a network analyzer. In both kinds of measurements, a very good agreement between calculated and measured values was found.

Reference articles

  • Calabrese M et al. (2006): [1.95 GHz周波数の高効率インビトロ導波管ばく露装置]
  • Zeni O et al. (2005): [900 MHzラジオ周波数電磁界への急性ばく露によるヒトリンパ球での遺伝毒性評価]
  • Kuster N et al. (2000): [無線通信に関係した健康リスクに着目した生物実験のばく露装置の最低限必要な条件とガイドライン]
  • Allis JW et al. (1977): [生物系に吸収されるマイクロ波放射の測定（1）。加熱および冷却データの分析]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

No statistically significant differences were found in continuously exposed and sham exposed human leukocytes concerning cell viability and genotoxicity.

研究の種別:

研究助成

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