この研究は、835MHz、90V/mの電磁界がヒト－ハムスターハイブリッド細胞株FC2細胞に生じさせる紡錘体異常を調べた。ばく露にはμTEM を用い、ばく露パラメータを正確に制御した。SARは数値計算し、最大で60 mW/kgであった。独立的に2回実験した。なお、温度の影響が除外されていることを実験的に確認した。その結果、対照群に比較して、90 V/mへの0.5－2 時間ばく露により、細胞分裂の後期（anaphase）、終期（telophase）に紡錘体異常が出現した；この紡錘体異常があっても、有糸分裂細胞の細胞周期分布には変化がなかった、と報告している。
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To evaluate in more detail (in comparison to a previous study, cf. publication 16115) the capacity of radiofrequency irradiation at a electric field strength of 90 V/m to act during a narrow time dependent window of the mitotic cycle, that is, the passage from metaphase to anaphase.
In the preliminary study on the induction of spindle disturbances in mammalian cells by radiofrequency exposure, the authors could demonstrate that with increasing electric field strength from 20 to 90 V/m there was an obvious tendency for an increase in the fraction of damaged anaphases and telophases but a less marked increase in the fraction of damaged metaphases.
Positive controls were exposed to trichlorfon.
| 周波数 | 835 MHz |
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| タイプ |
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| 特性 |
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| ばく露時間 | continuous for 0, 0.17, 0.5, 1, and 2 h |
| Modulation type | CW |
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| ばく露の発生源／構造 | |
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| チャンバの詳細 | A special exposure setup was used [Schmid and Schrader, 2007] that fulfilled the minimal requirements proposed by [Kuster and Schonborn, 2000] and allowed optimal experimental conditions for small biological samples. The µTEM cell itself was optimized to provide a very low VSWR close to unity, and standing waves were avoided as much as possible by the chosen setup. |
| ばく露装置の詳細 | The FC2 cells were grown as a monolayer in so-called slide flasks and were exposed at room temperature (20-22 °C) in a µTEM cell. |
| Additional information | Positive controls were exposed to trichlorfon for up to 6 h. |
The data suggest that an electric field strength of 90 V/m is a spindle acting agent in human-hamster hybrid cells as indicated by the appearance of spindle disturbances at the metaphase stage and preferably at the anaphase and telophase stages of cell divisions. The fraction of anaphases and telophases with spindle disturbances increased with increasing exposure time from a low spontaneous fraction to a saturation value of 20%.
It may be possible that electromagnetic fields induce biological effects in FC2 cells via non-thermal pathways. However, this finding does not necessarily mean that the induced effects lead to disease.
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