研究のタイプ: 医学/生物学の研究 (experimental study)

[携帯通信周波数範囲の信号がヒトーハムスタハイブリッド細胞に誘発した紡錘体異常] med./bio.

Spindle disturbances in human-hamster hybrid (AL) cells induced by mobile communication frequency range signals.

掲載誌: Bioelectromagnetics 2008; 29 (8): 626-639

この研究は、835MHz、90V/mの電磁界がヒト-ハムスターハイブリッド細胞株FC2細胞に生じさせる紡錘体異常を調べた。ばく露にはμTEM を用い、ばく露パラメータを正確に制御した。SARは数値計算し、最大で60 mW/kgであった。独立的に2回実験した。なお、温度の影響が除外されていることを実験的に確認した。その結果、対照群に比較して、90 V/mへの0.5-2 時間ばく露により、細胞分裂の後期(anaphase)、終期(telophase)に紡錘体異常が出現した;この紡錘体異常があっても、有糸分裂細胞の細胞周期分布には変化がなかった、と報告している。

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研究目的(著者による)

To evaluate in more detail (in comparison to a previous study, cf. publication 16115) the capacity of radiofrequency irradiation at a electric field strength of 90 V/m to act during a narrow time dependent window of the mitotic cycle, that is, the passage from metaphase to anaphase.

詳細情報

In the preliminary study on the induction of spindle disturbances in mammalian cells by radiofrequency exposure, the authors could demonstrate that with increasing electric field strength from 20 to 90 V/m there was an obvious tendency for an increase in the fraction of damaged anaphases and telophases but a less marked increase in the fraction of damaged metaphases.
Positive controls were exposed to trichlorfon.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 835 MHz
Modulation type: CW
ばく露時間: continuous for 0, 0.17, 0.5, 1, and 2 h

ばく露1

主たる特性
周波数 835 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 0, 0.17, 0.5, 1, and 2 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
チャンバの詳細 A special exposure setup was used [Schmid and Schrader, 2007] that fulfilled the minimal requirements proposed by [Kuster and Schonborn, 2000] and allowed optimal experimental conditions for small biological samples. The µTEM cell itself was optimized to provide a very low VSWR close to unity, and standing waves were avoided as much as possible by the chosen setup.
ばく露装置の詳細 The FC2 cells were grown as a monolayer in so-called slide flasks and were exposed at room temperature (20-22 °C) in a µTEM cell.
Additional information Positive controls were exposed to trichlorfon for up to 6 h.
パラメータ
測定量 種別 Method Mass 備考
電界強度 90 V/m effective value 計算値 - -
SAR 60 mW/kg maximum 計算値 - -

Reference articles

  • Schmid E et al. (2007): [哺乳類細胞における電離および非電離放射線の生物的な有効性はそれぞれ違っている]
  • Kuster N et al. (2000): [無線通信に関係した健康リスクに着目した生物実験のばく露装置の最低限必要な条件とガイドライン]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data suggest that an electric field strength of 90 V/m is a spindle acting agent in human-hamster hybrid cells as indicated by the appearance of spindle disturbances at the metaphase stage and preferably at the anaphase and telophase stages of cell divisions. The fraction of anaphases and telophases with spindle disturbances increased with increasing exposure time from a low spontaneous fraction to a saturation value of 20%.
It may be possible that electromagnetic fields induce biological effects in FC2 cells via non-thermal pathways. However, this finding does not necessarily mean that the induced effects lead to disease.

研究の種別:

研究助成

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