研究のタイプ: 医学/生物学の研究 (experimental study)

[弱いマイクロ波はコムギ苗での浸透圧ストレスにより生じる水分欠乏を緩和する] med./bio.

Weak microwave can alleviate water deficit induced by osmotic stress in wheat seedlings.

掲載誌: Planta 2009; 229 (2): 291-298

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研究目的(著者による)

To study the effect of microwave pretreatment on wheat seeds on the resistance of seedlings to osmotic stress and to evaluate the potential biological effects of low-level microwaves on oxidative damage (induced by osmotic stress).

詳細情報

High activities of antioxidant enzymes and high contents of non-enzymatic constituents (e.g. antioxidants) are important for plants to tolerate environmental stresses.
Six different doses of microwave exposure (0, 5, 10, 15, 20, 25 s) were tested to select the best dose for a following protective experiment (resistence to osmotic stress). In the protective experiment, wheat seeds were randomly divided into four experimental conditions: 1) control group, 2) microwave exposure, 3) osmotic stress: polyethylene glycol 600, 4) microwave exposure (10 s) + osmotic stress (polyethylene glycol 600). At least three replications were performed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
  • unspecified
ばく露時間: 5, 10, 15, 20 or 25 s
  • magnetic field intensity: 10 mH - 55 mH; measured 6 h after exposure

General information

seeds were divided into four groups: i) control - no treatment ii) exposure to microwaves for 10 s iii) osmotic stress iv) exposure to microwaves for 10 s + osmotic stress

ばく露1

主たる特性
周波数
  • unspecified
タイプ
  • electromagnetic field
ばく露時間 5, 10, 15, 20 or 25 s
ばく露装置
ばく露の発生源/構造
  • microwave oven
ばく露装置の詳細 seeds placed in the center of the microwave oven
パラメータ
測定量 種別 Method Mass 備考
参照 - - - - magnetic field intensity: 10 mH - 55 mH; measured 6 h after exposure

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The seed temperature increased progressively with microwave pretreatment dose compared to the control group. Each microwave pretreatment resulted in an increased α-amylase enzyme activity and biophoton emission intensity with a peak value at microwave pretreatment of 10 s.
In the seedlings with microwave exposure and osmotic stress (group 4), the concentration of malondialdehyde decreased, while the enzyme activities of nitric oxide synthase, catalase, peroxidase, superoxide dismutase and concentration of nitric oxide, ascorbic acid and glutathione increased.
The results suggest that a suitable dose of microwave exposure can enhance the capability of plants to eliminate free radicals and thus may alleviate water deficit induced by osmotic stress in wheat seedlings.

研究の種別:

研究助成

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