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To study the effects of radiofrequency electromagnetic field exposure on neuronal phenotype maturation in two different in vitro models: murine primary cortical neurons (of rat) and murine cholinergic SN56 cell line.
primary cortical neurons (of rat) and cholinergic SN56 cell line
分子生合成: mRNA expression of cytoskeleton regulating factors, e.g. beta-thymosin (linked to neurite outgrowth regulation), and of stress-related early response genes (c-Fos and c-Jun); semiquantitative Real-time PCR
形態学/組織学的変化: neuronal phenotype maturation (neurite length, number of neurites, sprouting, outgrowth and branching) (beta-tubulin staining; inverted microscopy and fluorescence microscopy
The data showed that radiofrequency electromagnetic field exposure reduced the number of neurites generated by both cell systems, and this alteration correlates to increased gene expression of beta-thymosin. Gene expression of c-Fos and c-Jun was not affected by exposure. Additionally, neither neurite length nor branching was affected by exposure. The alteration of beta-thymosin expression by radiofrequency exposure could be a direct or an indirect effect of the irradiation. Further studies are needed (also by means of other exposure schedules) to verify the effects described and to find out the molecular mediators and pathways modifying neural cell phenotype.