研究のタイプ: 医学/生物学の研究 (experimental study)

[キイロショウジョウバエの卵巣発生に対するマイクロ波ばく露の影響] med./bio.

Effect of microwave exposure on the ovarian development of Drosophila melanogaster.

掲載誌: Cell Biochem Biophys 2012; 63 (2): 121-132

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研究目的(著者による)

To study whether the decreased reproduction in insects (Drosophila melanogaster), recorded in authors' previous studies (see "Related articles") after GSM exposure, is accompanied by decreased ovarian development during the developmental period of the first eggs in the ovaries of virgin flies (oogenesis). Oogenesis starts at the late stages of pupation and lasts until about 48 h after eclosion. Furthermore, the authors aimed to verify whether the potential effects were caused by elimination of eggs during early and mid-oogenesis due to DNA damage and cell death induction.

詳細情報

Newly emerged flies were divided into seven groups according to the number of hours following eclosion when they were investigated/dissected (0, 10, 20, 32, 39, 45, 51 h). Additionally, all groups (except 0 h group) were divided into two subgroups: a sham exposed and an exposed subgroup (each group n=8).
For cell death investigation ovarioles were investigated (background information on ovarioles, see Panagopoulos 2007b).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 6 min., every 10 h (first exposure 3 h after eclosion), max. for 5 times

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • near field
ばく露時間 continuous for 6 min., every 10 h (first exposure 3 h after eclosion), max. for 5 times
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • セルラ-電話
チャンバの詳細 antenna of the cell phone in contact with the cylindrical glass vials containing the flies
ばく露装置の詳細 the mobile phone was held close to the experimenter's head with its antenna facing downward, being at the same time parallel to and in contact with the glass vials; the experimenter spoke to the mobile phone's microphone during connection ("modulated emission")
Sham exposure A sham exposure was conducted.
Additional information no temperature increases during the GSM exposures were recorded within the vials with the insects or within the mass of the food
パラメータ
測定量 種別 Method Mass 備考
SAR 0.795 W/kg maximum 備考を参照のこと。 - according to the manufacturer
電力密度 0.354 mW/cm² mean 測定値 - ± 0.063 mW/cm²
電界強度 17.5 V/m mean 測定値 - ± 3.2 V/m
磁束密度 0.08 µT mean 測定値 - ± 0.02 µT

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed that the ovarian size of the exposed insects was significantly decreased compared with the sham exposed insects. The difference in ovarian size between sham exposed and exposed virgin female flies became significant after two exposures (à 6 min) and was most evident 39-45 h after eclosion (max. decrease 29.75%) when the first eggs within the ovaries were in the stages of mid-late oogenesis.
Data on DNA fragmentation and cell death are not completely shown, but the authors declare that a large percent of DNA damage and cell death was found (average 54.42% TUNEL-positive cells in exposed insects, 6.98% in sham exposed).
In conclusion, the data showed that GSM exposure retarded ovarian development in Drosophila melanogaster and that these effects were caused probably due to a DNA damage and cell death induction in the egg chamber cells during early and mid-oogenesis.

研究の種別:

研究助成

関連論文