研究のタイプ: 医学／生物学の研究 (experimental study)

[弱い水平方向の超低周波磁界の影響下でのダイコン新芽の脂質利用] med./bio.

Lipid utilization in radish seedlings as affected by weak horizontal extremely low frequency magnetic field

著者: Novitskii YI, Novitskaya GV, Serdyukov YA
掲載誌: Bioelectromagnetics 2014; 35 (2): 91-99
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この研究は、超低周波磁界（周波数50 Hz、磁束密度～500 μT）下で、薄明中および暗闇中で生育させたダイコン（二十日大根）の5日齢の新芽における脂質の組成と含有量を調べた。対照群は、磁界以外を同一条件として生育させた。脂質代謝産物を種子の脂質組成と比較した。対照群では、貯蔵されていた中性脂質（大半はトリアシルグリセリド）が極性脂質（PL）形成に利用され、糖脂質（GL）およびリン脂質（PhL）を主体としたPLの量が、種子での含有量の2倍になった。それに対し、磁界ばく露群では、20-22℃において、薄明中生育では、PL産生は種子での3倍に増加した（GL含有量は4倍､PhL含有量は2.5倍）；暗闇中生育では、磁界の影響はこれより弱まった；13-16℃においては、薄明中では磁界の影響は弱まり、暗闇中では磁界の影響は全く見られなかった、と報告している。

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研究目的（著者による）

To evaluate the effects of an extremely low frequency magnetic field on the lipid synthesis in radish seeds during the seedling development under different temperature and illumination conditions.

詳細情報

The following groups were examined: 1.) control group with light, 2.) control group in the dark 3.) magnetic field exposure with light and 4.) magnetic field exposure in the dark. The experiment was performed two times. In the first run, the temperature was 20-22°C and in the second 13-16°C.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 5 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 5 days
ばく露装置
ばく露の発生源／構造
チャンバの詳細 seeds and seedlings were put in Petri dishes in the middle of the Helmholtz coils
ばく露装置の詳細 magnetic field produced by two pairs of Helmholtz coils (diameter 420 mm)
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 500 µT - 測定値 - -
Additional parameter details

control seedlings were also grown in Helmholtz coils but electric current was switched off, magnetic flux density was 38 µT and the vector of the magnetic field was directed to the horizon at an angle of 73°

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Under control conditions, the seedlings utilized reserve neutral lipids (mostly triglycerides) for the formation of polar lipids (two times higher in 5 day-old seedlings than in seeds). At 20-22°C in the light, the magnetic field exposure significantly increased the production of polar lipids (threefold compared to seeds), glycolipids and phospholipids in 5 day-old seedlings compared to the control group. However, in the darkness, the magnetic field exposure significantly decreased the production of those lipids.
At 13-16°C in the light, the content of polar lipids, glycolipids and phospholipids was slightly but significantly increased in the magnetic field exposed 5 day-old seedlings compared to the control group, while in the dark no significant difference occurred between the control group and the magnetic field exposure.
The authors conclude that magnetic field exposure stimulated the lipid synthesis in radish seedlings grown in the light, whereby this effect was stronger at higher temperatures.

研究の種別:

研究助成

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