研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波磁界(50Hz, 0.5mT)はショウジョウバエの適応度成分および活動度を加減する] med./bio.

Extremely low frequency magnetic field (50 Hz, 0.5 mT) modifies fitness components and locomotor activity of Drosophila subobscura.

掲載誌: Int J Radiat Biol 2014; 90 (5): 337-343

この研究は、ショウジョウバエ(Drosophila subobscura)の卵から初齢幼虫への発達期に48時間、超低周波磁界(ELF-MF;50 Hz, 0.5 mT)ばく露を行い、その後の幼虫適応度成分(発達時間、発達動態(日ごとの羽化成虫数)、生存率、成虫の性比)および3日齢の雄、雌の活動度を調べた。また別の実験で、羽化直後の成虫に48時間のELF-MFばく露を行い、ばく露終了直後に活動度を測定した。その結果、ELF-MFばく露は、発達時間を短縮し、生存率を高めたが、性比には影響しなかった;ばく露の時期に拘わらず、ELF-MFは成虫の活動度を有意に減少させたが、羽化直後の成虫へのばく露の方が、変化が長く続いた、と報告している。

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研究目的(著者による)

To investigate the effects of an exposure to a 50 Hz-magnetic field on Drosophila subobscura at different developmental stages.

詳細情報

In the experimental group, Drosophila subobscura was exposed as a larvae (egg-first instar larvae, n=75 per vial) or as just eclosed adults (n=35). Additionally, a control group (n=20) without any treatment and a sham exposed group (egg: n=75, adult: n=75) were examined in the same developmental stages.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 48 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 48 hours
ばく露装置
ばく露の発生源/構造
チャンバの詳細 larvae were exposed in petri dishes, adult flies were exposed in 60 m3 vials
ばく露装置の詳細 magnetic field was generated by electromagnet consisting of three circular coils (37 cm diameter) of insulted copper wire (0.75 mm diameter); distance of coils to each other 23 cm, homogeneous magnetic field in a horizontal direction; no observed fluctuations in temperature or vibrations
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - ± 0.01 mT

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The results of the control without treatment and the sham exposed group showed no significant differences. Hence, they were cumulated and used as one control group.
No difference in the sex ratio between exposed and control flies was observed.
The development of the flies was significantly faster in the exposure group compared to the control group. Both, starting and finishing of eclosing took place one day earlier in the exposure group than in the control group. Additionally, in exposed flies the viability was significantly increased compared to the control group.
In both developmental stages, the locomotor activity of exposed flies was significantly increased in comparison to the control group.
The authors conclude that exposure to a 50 Hz-magnetic field could influence the viability, development and locomotor activity of Drosophila subobscura.

研究の種別:

研究助成

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