研究のタイプ: 医学/生物学の研究 (experimental study)

[ラットにおける血清パラオキソナーゼ活性および脂質過酸化代謝に対する超低周波電磁界の影響] med./bio.

Effects of extremely low frequency electromagnetic fields on paraoxonase serum activity and lipid peroxidation metabolites in rat.

掲載誌: J Diabetes Metab Disord 2014; 13 (1): 85 - 1-85 - 9

この研究は、超低周波磁界(60Hz、0.5mT:ヘルムホルツコイルばく露により抗酸化機能障害と脂質過酸化上昇が生じて、アテローム生成的な影響が起きるのではないかという仮説の下、ラット実験でパラオキソナーゼ(PON)活性、抗酸化能、脂質過酸化代謝への影響を調べた。75匹のウィスターラットを5群に分けた:擬似ばく露群、一回ばく露群(血液検査:ばく露直後)一回ばく露群(ばく露の72時間後)、14日間ばく露群(直後)、14日間ばく露群(72時間後)。その結果、一回ばく露群では、脂質過酸化、PONを含む血清酸化活性の上昇が有意であった;慢性ばく露では、脂質過酸化が上昇、抗酸化系が影響を受けた、などの所見を報告している。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的(著者による)

The effects of a whole-body exposure of rats to a 60 Hz magnetic field on factors in blood which can provoke atherosclerosis should be investigated.

詳細情報

75 Wistar rats were randomly assigned to five groups: 1) sham exposure, 2) 4 h exposure, sacrificed immediately thereafter, 3) 4 h exposure, sacrificed 72 h thereafter, 4) 14 days of exposure, sacrificed immediately thereafter, and 5) 14 days of exposure, sacrificed 72 h thereafter. Blood samples were taken directly after sacrificing and analyzed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 4 hours
single exposure
ばく露2: 60 Hz
ばく露時間: continuous for 14 days
chronic exposure

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 hours
Additional information single exposure
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 A pair of Helmholtz coils set with winding surrounded in a plexi-glass frame. Through a variable transformer the coils were connected to a 220 V AC power supply. The centrally horizontal magnetic field was homogenous in a plexi-glass cage.To adjust the frequency and intensity, the coils were linked to a waveform generator.
Sham exposure A sham exposure was conducted.
Additional information Sham exposure group was placed in the same cage though the coils were turned off, being exposed to the local geomagnetic field (B-field, horizontal component = 0.018 mT, B-field vertical component = 0.022 mT, B-field total = 0.023 mT and B-field alternating current < 0.002 mT).
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - -

ばく露2

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 14 days
Additional information chronic exposure
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - -

Reference articles

  • Lee BC et al. (2004): [マウス脳の抗酸化防御系への低周波磁界の影響:化学発光研究]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The results of all measured parameters of groups 2-5 were compared to the results of the sham exposure (group 1), respectively. A significantly higher paraoxonase enzyme activity, higher levels of conjugated diens, high density lipoprotein and free fatty acids as well as a significantly lower total antioxidative capacity could be observed in group 2 directly after the 4 h exposure. However, 72 h after the 4 h exposure (group 3), no significant alterations could be observed.
For group 4, significant changes of parameters comparable to the results of group 2 could be observed directly after 14 days of exposure, but additionally the level of malondialdehyde was significantly higher. 72 h after the 14-days exposure, the paraoxonase enzyme activity and the level of malondialdehyde were significantly higher and the total antioxidative capacity significantly lower (group 5).
The authors conclude that already a single exposure to a 60 Hz magnetic field with 0.5 mT has significant effects on factors in rat blood which can provoke atherosclerosis. However, these effects are reversible. The effects of a chronic exposure seem to be even more distinct and tend to be not reversible.

研究の種別:

研究助成

関連論文