研究のタイプ: 医学/生物学の研究 (experimental study)

[マウス骨髄細胞の造血系細胞への分化能に対するGSM変調900MHz無線周波電磁界の影響] med./bio.

Effects of GSM-modulated 900 MHz radiofrequency electromagnetic fields on the hematopoietic potential of mouse bone marrow cells.

掲載誌: Bioelectromagnetics 2014; 35 (8): 559-567

この研究は、2つのマウス移植モデルを用いて、骨髄細胞(BMC)に対するGSM変調900MHz無線周波電磁界RFばく露の影響を調べた。第一のモデルは、RFばく露マウスおよび疑似ばく露マウスのどちらか一方のBMCをX線照射した同系マウスに移植した。その結果、移植から12週間後、どちらのBMCの移植を受けたマウスも、胸腺細胞の数、細胞亜群および細胞増殖の頻度に差がなかった。第二のモデルは、RFばく露および擬似ばく露した類遺伝子型マウスの両方のBMCを混合して、リンパ球減少したRag2欠損マウスに同時移植した。その結果、Rag2欠損マウスのリン器官での成熟リンパ球補充競争において、移植したBMCは有利にも不利にも働かなかった、と報告している。

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研究目的(著者による)

The ability of bone marrow precursor cells from mice, exposed to a radio frequency field, to colonize thymus and spleen of lethally x-irradiated mice and to differentiate should be investigated. The study is an expansion to the precursor study of Prisco et al., 2008

詳細情報

The study should verify the results of the precursor study and check them with the help of a new, sensitive method. Therefore, two different approaches were conducted: In approach 1, lethally x-irradiated wild type B/6 mice received injections with bone marrow cells from syngenic exposed (1a, n=8), sham exposed (1b, n=8) and cage control animals (1c, n=8). In approach 2, three X-irradiated Rag2 deficient mice received 50:50 mixtures of bone marrow cells: 2a) from cage control (wild type B/6) and sham exposure (congenic B/6)), 2b) from sham exposure (congenic B/6) and exposure (wild type B/6) and 2c) from cage control (congenic B/6) and exposure (wild type B/6).
As Rag2 deficient mice cannot produce mature B cells and T cells, approach 2 was used to detect differences between two different treatments, respectively, with a very high sensitivity, as those cells develop from the transplanted bone marrow cells, compete in the colonization of thymus and spleen and their origin can be tracked. Wild type and congenic donor mice express different variants of the Thy1 surface molecule CD90 on T cells (Thy1a und Thy1b) and of the immunoglobulin heavy chain Igh on B cells (Igha und Ighb), which can be detected with specific antibodies. Investigation took place 12 weeks after transplantation.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 2 h/day and 5 days/week for 4 weeks

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 2 h/day and 5 days/week for 4 weeks
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源/構造
  • セルラ-電話
チャンバの詳細 8 mice each were placed in a Transverse ElectroMagnetic (TEM) chamber (12 x 12 x 120 cm) made of transparent cylindrical polymethyl methacrylate
ばく露装置の詳細 mice were fixed with their caudal axis parallel to the direction of the field propagation and exposed with daily clockwise rotation of the animals
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg - 測定値および計算値 - -

Reference articles

  • Laudisi F et al. (2012): [無線周波への出生前ばく露:動物モデルの胸腺細胞発達および末梢T細胞コンパートメントに対するWiFi信号の影響]
  • Prisco MG et al. (2008): [マウスの骨髄細胞に対するGSM変調無線周波電磁界の影響]
  • Ardoino L et al. (2005): [電磁界の生物学的影響の研究を目的としたIn vivoパイロット試験用の無線周波システム]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

No differences regarding total thymocyte and spleen cell numbers, percentages of subpopulations, cell proliferation and level of interferon-gamma could be found between the groups of test approach 1. Approach 2 revealed a slight but insignificant higher amount of cells stemmed from the cage control animals in mice 2a) and 2c). This was interpreted as a decreased fitness of cells from sham exposure and exposure animals due to the increased stress they had encountered during the exposure procedure.
The authors conclude that their results indicate no effects of a chronic whole body exposure to a 900 MHz GSM electromagnetic field on the ability of bone marrow precursor cells from mice to colonize thymus and spleen and to differentiate and thus that the results of their precursor study are verified.

研究の種別:

研究助成

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