研究のタイプ: 医学/生物学の研究 (experimental study)

Effects of continuous exposure to power frequency electric fields on soybean Glycine max. med./bio.

掲載誌: J Environ Radioact 2019; 204: 35-41

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研究目的(著者による)

To study the effects of exposure of soybean plants to 50 Hz electric fields on various parameters such as growth and nutrient content.

詳細情報

Soybeans (Glycine max) were sown under identical conditions and divided into 3 groups: 1) exposure to a 2 kV/m electric field, 2) exposure to a 10 kV/m electric field and 3) control group. Ten plants were randomly selected from each group and investigated every 10 days. An age up to 40 days was defined as the seedling stage, 40-60 days as the flowering stage and an age of more than 60 days as the maturity stage.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuously for up to 80 days
ばく露2: 50 Hz
ばく露時間: continuously for up to 80 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • electric field
ばく露時間 continuously for up to 80 days
ばく露装置
ばく露の発生源/構造
  • electrode
チャンバの詳細 three seedlings per round plastic pot (30 cm diameter × 30 cm height); an interval of 1 m was kept between pots
ばく露装置の詳細 the exposure system consisted of a metal mesh plate 2.6 m above ground level, with insulating pillars that supported the power supply, voltage regulator and transformer; field inhomogeneity was less than 5% and an interval of more than 2 m was maintained between each exposure/control area to eliminate vibration and mutual interference; to avoid edge effect, plants in the middle of each exposure area were selected for sampling
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 2 kV/m - - - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • electric field
ばく露時間 continuously for up to 80 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 10 kV/m - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The chlorophyll content of soybean leaves was significantly higher in the 2 kV/m group (group 1) than in the 10 kV/m group (group 2) and the control group (group 3) at the seedling and flowering stages. The heights of soybean plants in both exposure groups were significantly higher than those in the control group. At the seedling stage, the stem diameter of plants in group 1 was significantly higher than that of plants in group 2 and the control group. The germination rate increased significantly with intensity of the electric field.
The soluble sugar, total protein and total amino acid contents were significantly increased in group 1 compared to the control group during seedling stage and partly also during later stages and in comparison to group 2. The enzyme activities of all investigated enzymes were significantly decreased in the exposure groups 1 and 2 compared to the control group, mostly during the seedling stage.
In the transcriptome analysis of group 2, 2,977 genes were significantly up-regulated and 1,462 genes were down-regulated compared to the control group. The differentially expressed genes mainly encoded ribosome proteins and related enzymes involved in carbon metabolism pathway, what might provide insights into the underlying mechanisms of action.
The authors conclude that exposure of soybean plants to 50 Hz electric fields affects various parameters such as growth and nutrient content, especially in low intensity fields and exposures during seedling stage of the plants.

研究の種別:

研究助成

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