electrophoretic mobility shift assay

  • EMSA
  • gel mobility shift assay
  • gel retardation assay
German: Electrophoretic mobility shift assay
Japanese: 電気泳動移動度シフト解析

Molecular genetics. A lab technique used to find out if there are proteins binding a fragment of DNA. It is based on the

observation that peptide-DNA complexes migrate through polyacrylamide gels

more slowly than free DNA fragments because larger complexes are more retarded than smaller ones. This assay is for example used for the identification of sequency-specific DNA-binding proteins as well as transcription factors.

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