To study the effects of low frequency magnetic field exposure on the initial stages of germination of wheat seeds. As sensitive parameters to the action of a low frequency electromagnetic field, besides the biological indices (germinating power, rate of germination, etc.), the authors chose change in pH in the direct vicinity of the seed germ and change in the hydrolytic activity of the esterase enzymes released at the early stages of germination of wheat seeds.
In an interval between one and 30 h after the start of wetting the plant samples were exposed to the magnetic field. Different wheat varieties of different ages were used.
Exposure duration: continuous for 7 min to 10 min
|Exposure duration||continuous for 7 min to 10 min|
|Setup||petri dishes with seeds placed on the surface of the mixer|
|magnetic flux density||30 mT||-||-||-||-|
Brief magnetic field exposure of the wheat seeds in the first hours of swelling had relatively little influence on the esterase enzyme activity. However, at the stage of growth in seeds with high germinating power, the magnetic field effect (increased enzyme activity) sharply increased by a factor of two and more in individual experiments. After 11 h, strong effects were noted in all tests and, starting from hours 17-18, the magnetic field only weakly influenced enzyme activity. At the same time, in old seeds, the magnetic field exposure either did not change or lowered esterase enzyme activity.
In some experiments the magnetic field exposure produced an earlier pH value shift close to the germ surface (acceleration of cell acidification process).
The germinating power and the rate of germination also sharply increased following magnetic field exposure.
The authors conclude that the data point out the high sensitivity of wheat seed in the course of their germination to exposure to a low frequency magnetic field.