Study type: Medical/biological study (experimental study)

Effects of time-varying magnetic fields on fibroblast growth med./bio.

By: Schuetz PW, Barbenel JC, Paul JP
Published in: Clin Phys Physiol Meas 1985; 6 (2): 155-160
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Aim of study (acc. to author)

To investigate the effects of time-varying magnetic fields at frequencies of 10 and 27 MHz on the growth of mouse L929 fibroblast cells .

Background/further details

The criteria to determine the number of dividing fibroblast cells were that these cells become detached from the substrate and appear rounded instead of their typical shape.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 10 MHz
Exposure duration: 2 h, 4 times a day (number of days not given)
Exposure 2: 27 MHz -

Exposure 1

Main characteristics
Frequency 10 MHz
Type
Exposure duration 2 h, 4 times a day (number of days not given)
Exposure setup
Exposure source
Chamber 60 ml petri dishes filled with 10ml Eagles's MEM without NEAA
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 64.5 µT - - - -

Exposure 2

Main characteristics
Frequency 27 MHz
Type
Exposure setup
Parameters

No parameters are specified for this exposure.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposed cells showed a greater number of cell division and reached their maximum earlier as unexposed cells. Furthermore exposed cells showed significant changes in morphology and growth orientation. Most cells displayed a modified shape, large nucleus, lipid inclusions and prominent Golgi apparatus; finally exposed cells became radial oriented and grew in multilayers in a cluster formation.

Study character:

Study funded by