To investigate the possible alterations of proliferation and ATP level in cultures of the bacterium Corynebacterium glutamicum during relatively long time of EMF exposure.
Exposure took place with a varieties of amplitudes and frequencies.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
10–70 Hz
Exposure duration:
continuous upto 8 h
|
|
|
Exposure 2:
10–70 Hz
Exposure duration:
continuous upto 8 h
|
|
| Frequency | 10–70 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous upto 8 h |
| Exposure source | |
|---|---|
| Setup | A vessels of cell suspension was maintained at contant temperature (28°C) surrounded by coils |
| Additional info | Another vessel was used for control. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 3.4 mT | mean | measured | - | - |
| Frequency | 10–70 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous upto 8 h |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 4.9 mT | mean | measured | - | - |
Two particular windows were found: At a frequency of 15 Hz and an amplitude of 3.4 mT the ATP level increased more than 20% compared with the control experiment after 8 h of continuous exposure. At a frequency of 50 Hz and an amplitude of 4.9 mT the ATP level was increased more than 30% compared to the control experiment after 6 h of continuous exposure. The cell proliferation was stimulated in the same direction as the ATP level, thus the ATP concentration per cell remained constant.
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