Study type: Medical/biological study (experimental study)

Japanese encephalitis virus (JEV): potentiation of lethality in mice by microwave radiation med./bio.

By: Lange DG, Sedmak J
Published in: Bioelectromagnetics 1991; 12 (6): 335-348
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Aim of study (acc. to author)

To demonstrate that the effect of microwave radiation on blood-brain barrier function is due to an alteration in capillary endothelial cell permeability.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: intermittent; 10 min/day, days 1,2,4,8 after JEV or 4 consecutive days before JEV (japanese encephalitis virus)

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Waveform
Charakteristic
  • guided field
Polarization
Exposure duration intermittent; 10 min/day, days 1,2,4,8 after JEV or 4 consecutive days before JEV (japanese encephalitis virus)
Additional info References: Ho et al., 1973; Christman et al., 1974
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber waveguide 5.4 x 10.9 x 27cm, TE10 mode, styrofoam containment cage 5 x 9 x 11cm with 0,6 cm diameter lucit rods
Setup animals were placed unanesthetized and unrestrained in the styrofoam cage
Parameters
Measurand Value Type Method Mass Remarks
power density 200 W/m² unspecified measured - corresponding SAR: 45.5 W/kg calculated by means of Dewar-flask calorimetric technique
power density 500 W/m² unspecified measured - corresponding SAR: 97.7 W/kg calculated by means of Dewar-flask calorimetric technique
power density 250 W/m² unspecified measured - corresponding SAR: 54.2 W/kg calculated by means of Dewar-flask calorimetric technique
power density 100 W/m² unspecified measured - corresponding SAR: 23.7 W/kg calculated by means of Dewar-flask calorimetric technique

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Exposure to microwaves resulted in a dose-dependent increase in Japanese encephalitis virus (JEV)-induced lethality. Increased amount of horseradish peroxidase (HRP) reaction product traversed the CNS capillary endothelial cells and entered brain parenchyma. HRP staining product was found around pericytes, basal lamina and within endothelial cells. It is not excluded that the increased JEV lethality may be due to alteration in immunologic status.

Study character:

Study funded by