Study type: Medical/biological study (experimental study)

Influence of 50 Hz electromagnetic fields on recurrent embryogenesis and germination of cork oak somatic embryos med./bio.

Published in: Plant Cell Tissue Organ Cult 1998; 54 (1): 65-69

Aim of study (acc. to author)

Isolated somatic embryos of Quercus suber were chronically exposed to a 50 Hz and 15 µT electromagnetic field in order to study if the extremely low frequency magnetic field affected the mophogenic behaviour of embryonic cultures (proliferation of embryonic cultures, differentiation of somatic embryos, and germination of somatic embryos) during recurrent embryogenesis. Additionally, germination of somatic embryos was carried out under the same electromagnetic field, and also under conditions in which the local geomagnetic field was suppressed.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 8 weeks
Exposure 2:
Exposure duration: continuous for 8 weeks
-

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 8 weeks
Exposure setup
Exposure source
Setup Somatic embryos were placed into open glass wells which were placed in closed Magneta vessels. Plastic trays containing Magneta vessels were placed in the geomagnetic center of the coils.
Additional info The applied field was perpendicular to geomagnetic field.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 15 µT effective value measured - -

Exposure 2

Main characteristics
Frequency
Type
Exposure duration continuous for 8 weeks
Exposure setup
Exposure source
Setup Helmholtz coils were arranged with their axis parallel to the total geomagnetic field (43 µT). A dc current was applied to the coils and regulated so that the generated field was opposed and had the same magnitude as the geomagnetic field.
Parameters

No parameters are specified for this exposure.

Reference articles

  • Picazo ML et al. (1996): Changes in mouse adrenalin gland functionality under second-generation chronic exposure to ELF magnetic fields. I. males.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The extremely low frequency magnetic field did not influence the growth of embryonic clumps of the assayed genotypes, but reduced the number of detachable embryos. The extremely low frequency magnetic field did not change the percentages of germination or plant formation of somatic embryos. Somatic embryos had better germination when cultured under the suppressed geomagnetic field condition.

Study character:

Study funded by

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