To determine the appropriate exposure time of an inhomogeneous high magnetic field able to supress the cell death of the bacterium Escherichia coli. Cell growth and death were investigated in special gene-deficient E. coli strain ass well.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
Exposure duration:
up to 72 h
|
|
| Frequency | |
|---|---|
| Type | |
| Waveform |
|
| Exposure duration | up to 72 h |
| Additional info | Inhomogeneous magnetic field with a maximum gradient of 23 T/m was applied |
| Exposure source | |
|---|---|
| Chamber | Incubator maintained at 37°C |
| Setup | A flask containing bacterial cells was placed in the region where the magnetic field was inhemogeneous |
| Additional info | For control purpose, an incubator was covered with a double-walled cylindrical shell of permalloy to bring the magnetic field lower than the geomagnetic field |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 5.2 T | minimum | unspecified | - | to 6.1 T |
E. coli B showed a drastic cell death after the end of the logarithmic growth phase, when grown aerobically at 43°C in an fourfold diluted standard medium containing 1.5 g/L glutamic acid. This observed cell death was dramatically suppressed by the use of an inhomogeneous high magnetic field. (q.v. publication 10598)
A twelve hour exposure to an inhomogeneous high magnetic field after the logarithmic growth phase were found to be sufficient to evoke the effect of cell death suppression. This magnetic field effect was not found on the rpoS- gene-deficient KT1005 E. coli strain.
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