Study type: Medical/biological study (experimental study)

Enzymatic kinetic change of ascorbate oxidase loaded into liposomes induced by microwave fields exposure med./bio.

By: Ramundo-Orlando A, Arcovito C, Palombo A, Serafino A, Mossa G
Published in: J Liposome Res 1993; 3 (3): 717-724
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Aim of study (acc. to author)

To study enzymatic kinetic change of enzyme ascorbate oxidase loaded into liposomes during microwave irradiation at temperature of 25°C.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Exposure duration: 3 min
  • SAR: 2.8 W/kg unspecified (unspecified)
Exposure 2: 2.45 GHz
Exposure duration: 3 min
  • SAR: 5.6 W/kg unspecified (unspecified)

Exposure 1

Main characteristics
Frequency 2.45 GHz
Exposure duration 3 min
Modulation
Modulation type unspecified
Exposure setup
Exposure source
  • not specified
Parameters
Measurand Value Type Method Mass Remarks
SAR 2.8 W/kg unspecified calculated unspecified -
Additional parameter details

SAR determined from the rate of temperature rise during the exposure.

Exposure 2

Main characteristics
Frequency 2.45 GHz
Exposure duration 3 min
Modulation
Modulation type unspecified
Exposure setup
Exposure source
  • not specified
Parameters
Measurand Value Type Method Mass Remarks
SAR 5.6 W/kg unspecified calculated unspecified -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • during exposure

Main outcome of study (acc. to author)

At both SARs the free enzyme in solution did not show any effects induced by microwave irradiation. The enzyme loaded into liposomes exhibits a significant decrease of 13% in the reaction velocity induced by microwave exposure at 5.6 mW/g. At SAR of 2.8 mW/g no significant decrease was observed.
The data demonstrate that microwave exposure could influence functional properties of ascorbate oxidase only when this enzyme is loaded into lipidic bilayer.

Study character:

Study funded by

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