The effects of distilled water exposed to extremely low frequency magnetic fields or extremely low frequency vibrations on barley seed hydration and germination should be investigated.
Distilled water was non-exposed (control), exposed to extremely low frequency magnetic fields (with frequencies of 4, 10,15, 20 or 50 Hz, no data regarding the strength of the magnetic field) or extremely low frequency vibrations (with frequencies of 4, 10, 15, 20 or 50 Hz and intensity of 30 dB) for 30 minutes prior to the start of the experiment. Afterwards, 20 barley seeds were incubated in warm (20°C) or cold (4°C), exposed or non-exposed water for five different durations (0, 2, 24, 48 and 72 h), respectively. Each approach was repeated 10 times. In a second stage of the experiment, seedlings were investigated over 18 days in non-exposed and exposed water. Approaches with cold water should account for passive uptake of water by depressed metabolism and approaches with warm water should account for active, metabolism-dependent water uptake.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
4–50 Hz
Exposure duration:
continuous for 30 min
|
- |
| Frequency | 4–50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 30 min |
| Exposure source | |
|---|---|
| Chamber | glass test tubes with diameter of 10 mm and volume of 10 ml were used |
| Setup | 106 mm high coils with a diameter of 154 mm and a separation of 77 mm located coaxially and parallel forming a Helmholtz ring |
No parameters are specified for this exposure.
No distinct differences in all investigated parameters could be detected in test approaches in cold water (passive uptake of water) between control and exposures to the magnetic fields or vibrations. In the approaches in warm water (active uptake of water), significant differences in dry weight, hydration and root formation could be found between control and the exposures to the magnetic fields or vibrations. These indicated partly activating and partly inactivating window effects regarding the frequency, which were different for both types of exposure.
The authors conclude that an exposure of distilled water to extremely low frequency magnetic fields or extremely low frequency vibrations has different effects on the metabolism-dependent processes of water uptake and growth in barley seeds and seedlings.
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