To study the effects of a physiological solution exposed to extremely low frequency electromagnetic fields, extremely low frequency electromagnetic field-modulated millimeter waves, or ionizing radiation on water absorption and dry mass changes in winter wheat seeds.
5 ml of an exposed physiological solution was added to each Petri dish containing 10 seeds and incubated for 2 h or 72 h. Dry and wet mass was determined before and after incubation of the seeds.
After 2 h incubation, the seed's metabolic activity is still in an inactive state and its water absorption depends on osmotic gradients between the seed and the culture medium (physiological solution), while after 72 h incubation, when germination processes are active, the seed hydration depends on anti-gradient water absorption by seeds also.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
Exposure duration:
continuous for 30 min
|
- |
|
Exposure 2:
14–16 Hz
Exposure duration:
continuous for 15 min
|
- |
|
Exposure 3:
90 MHz–160 GHz
Modulation type:
FM
Exposure duration:
continuous for 15 min
|
|
| Frequency | |
|---|---|
| Type | |
| Exposure duration | continuous for 30 min |
| Exposure source |
|
|---|
No parameters are specified for this exposure.
| Frequency | 14–16 Hz |
|---|---|
| Type | |
| Exposure duration | continuous for 15 min |
| Additional info | 14, 15, 16 Hz |
| Exposure source | |
|---|---|
| Setup | glas tube with a diameter of 10 mm and a volume of 10 ml placed between a pair of Helmholtz coils |
No parameters are specified for this exposure.
| Frequency | 90 MHz–160 GHz |
|---|---|
| Type | |
| Exposure duration | continuous for 15 min |
| Exposure source |
|
|---|---|
| Distance between exposed object and exposure source | 1 mm |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| power density | 1.8 mW/cm² | - | - | - | - |
After 2 h incubation in the exposed physiological solution the water absorption of the seeds was decreased as compared to the controls in unexposed medium, while after 72 h incubation the effect was reversed. The data suggest that the culture medium may be a target for the effects of non-ionizing radiation and ionizing radiation.
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