60 rats were used: 24 animals underwent unilateral sciatic nerve transection injury and immediate surgical nerve repair, 24 animals underwent unilateral sciatic nerve crush injury, and the remaining 12 animals underwent a sham surgery. Half of the rats (n = 12) with either sciatic nerve lesion were randomly chosen and irradiated for three weeks post-injury. The other half of the rats (n = 12) and six rats with sham surgery were used for two separate controls.
Functional recovery was followed for six weeks for the crush injuries and seven and a half months for the transection injuries.
|Chamber||cage placed within the coils system|
|Setup||rats placed in a round, non-conductive plastic cage (28 cm in diameter) and were free to move in it; the longitudinal rat`s axis was normally perpendicular to the magnetic field.|
|Sham exposure||A sham exposure was conducted.|
|Additional info||The generator producing the sine waves was interupted with a frequency of 0.45 Hz, hence creating an active and inactive period of 1.4 s and 0.8 s, respectively.|
|magnetic flux density||500 µT||unspecified||measured||-||-|
The positive effects of the stimulation on the functional recovery patterns in exposed animals and both injury models were statistically confirmed. Significant differences between exposed and control rats were revealed early at the start of functional recovery and later on from the points adjacent to the beginning of the functional recovery plateau until the end of observation.
These differences probably reflect the interrupted sinusoidal low frequency magnetic field systemic effect on the neuron cell bodies and increased (and more efficient) reinnervation of the periphery.