A matrix depleted cartilage explant model was used. Cartilage explants were obtained from metacarpophalangeal joints of calves and adult cows. Depletion of the extracellular matrix was performed by trypsin digestion. Digested tissue samples were divided into an exposure test group and non-exposure control group and were incubated with and without the addition of interleukin 1beta (it is thougth that interleukin 1beta plays a major role in cartilage destruction).
|Additional info||Cartilage explants were digested using trypsin and cultured in serum free basal medium with or without addition of interleukin 1β. The cultures were divided into a non-electromagnetic field group and an EMF exposure group. Undigested and untreated explants served as negative controls.|
|magnetic flux density||40 µT||unspecified||unspecified||-||-|
Electromagnetic field exposure promoted matrix macromolecule biosynthesis in intact explants (non-digested negative control) but had no stimulatory effect on damaged articular cartilage. The suppressive effects of interleukin 1beta were partially counteracted by exposure, exclusively in cartilage derived from young calves.
Electromagnetic field exposure has age dependant chondroprotective but not structure modifying properties when cartilage integrity is compromised. Thus, electromagnetic fields may not be useful as a therapeutic measure under these conditions.