Study type: Medical/biological study (experimental study)

Differentiation of Human Umbilical Cord-derived Mesenchymal Stem Cells, WJ-MSCs, into Chondrogenic Cells in the Presence of Pulsed Electromagnetic Fields. med./bio.

Published in: In Vivo 2013; 27 (4): 495-500

Aim of study (acc. to author)

To examine the possibility to enhance the cell differentiation of human mesenchymal stem cells through pulsed extremely low frequency magnetic fields.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 75 Hz
Exposure duration: 8 hours/day for up to 21 days

Exposure 1

Main characteristics
Frequency 75 Hz
Type
Waveform
Exposure duration 8 hours/day for up to 21 days
Additional info square wave
Exposure setup
Exposure source
Chamber cells were cultured in six-well culture plates and placed inside an incubator at equal distance from two solenoids
Setup solenoids were arranged in parallel with one another and perpendicular to the support base
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 3 mT maximum - - -
magnetic flux density 1.8 mT minimum - - -

Reference articles

  • Esposito M et al. (2012): Differentiation of human osteoprogenitor cells increases after treatment with pulsed electromagnetic fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Morphological changes indicating differentiation into cartilage cells were more evident and earlier in cells treated with pulsed magnetic fields compared with the untreated ones. The expression of typ II collagen and the secretion of proteoglycans were significantly increased in the exposed cell cultures compared to the untreated cell cultures.
The authors conclude that exposure of human mesenchymal stem cells to pulsed extremely low frequency magnetic fields could enhance cell differentiation into cartilage cells.

Study character:

Study funded by

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