Study type: Medical/biological study (experimental study)

Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field. med./bio.

Published in: J Environ Biol 2007; 28 (4): 735-740

Aim of study (acc. to author)

This study was performed to study biophoton charcteristics (ultraweak photon emission) of canine kidney cells under the influence of hydrogen peroxide with and without exposure to extremely low frequency magnetic field.

Background/further details

Biophoton is a photon of light emitted from a biological system (ultra low chemiluminescence), either generated spontaneously or as result of a biochemical cellular reaction, e.g. reactive oxygen species related metabolic activity. Hydrogen peroxide was applied in concentrations of 1, 2, 3, and 4 mM to generate reactive oxygen species. Biophoton measurement was performed by using both fluorescence microscopy and photomultiplier to examine whether these are reliable methods to detect biophton emission in living cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous from 2 h up to 3 h 40 min

General information

Cells were treated with different concentrations of H2O2 and then exposed to a magnetic field or not exposed.

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous from 2 h up to 3 h 40 min
Exposure setup
Exposure source
  • not specified
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.4 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:

Main outcome of study (acc. to author)

Both methods, fluorescence microscopy and photomultiplier, could detect increasing photon intensity due to hydrogen peroxide. The increase of the biophoton was dependent upon the amount of hydrogen peroxide. The decay of chemiluminescence intensity which was observed after hydrogen peroxide treatment alone showed a peak in the initial phase after additional magnetic field exposure.
The authors conclude that photomultiplier measurement might be a useful tool for studying biochemical characteristics in relation to reactive oxygen species.

Study character:

Study funded by