This study was performed to study biophoton charcteristics (ultraweak photon emission) of canine kidney cells under the influence of hydrogen peroxide with and without exposure to extremely low frequency magnetic field.
Biophoton is a photon of light emitted from a biological system (ultra low chemiluminescence), either generated spontaneously or as result of a biochemical cellular reaction, e.g. reactive oxygen species related metabolic activity. Hydrogen peroxide was applied in concentrations of 1, 2, 3, and 4 mM to generate reactive oxygen species. Biophoton measurement was performed by using both fluorescence microscopy and photomultiplier to examine whether these are reliable methods to detect biophton emission in living cells.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
60 Hz
Exposure duration:
continuous from 2 h up to 3 h 40 min
|
|
Cells were treated with different concentrations of H2O2 and then exposed to a magnetic field or not exposed.
| Frequency | 60 Hz |
|---|---|
| Type | |
| Exposure duration | continuous from 2 h up to 3 h 40 min |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1.4 mT | - | - | - | - |
Both methods, fluorescence microscopy and photomultiplier, could detect increasing photon intensity due to hydrogen peroxide. The increase of the biophoton was dependent upon the amount of hydrogen peroxide. The decay of chemiluminescence intensity which was observed after hydrogen peroxide treatment alone showed a peak in the initial phase after additional magnetic field exposure.
The authors conclude that photomultiplier measurement might be a useful tool for studying biochemical characteristics in relation to reactive oxygen species.
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