Study type: Medical/biological study (experimental study)

Response of the regulatory oscillatory behavior of copperII-containing ECTO-NOX proteins and of CuIICl2 in solution to electromagnetic fields. med./bio.

Published in: J Inorg Biochem 2008; 102 (9): 1812-1818

Aim of study (acc. to editor)

To study the effects of electromagnetic fields on the regulatory oscillatory behavior of growth and redox-related copperII-containing ECTO-NOX proteins and of CuIICl2 in solution.

Background/further details

Some ECTO-NOX proteins appear to function as core oscillators of the cells' biological clock. ECTO-NOX proteins catalyze two enzymatic activities: hydroquinone and NADH oxidation and disulfide-thiol interchange, alternating with a regular period length. ECTO-NOX proteins carry out hydroquinone (NADH) oxidation for 12 min (with two maxima separated by 6 min) and then that activity rests. While the hydroquinone (NADH) oxidative activity rests, the proteins engage in disulfide-thiol interchange activity for 12 min (with three maxima separated by intervals of 4.5 min). That activity then rests and the cycle repeats. Both reactions require copper.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 2 min

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 2 min
Exposure setup
Exposure source
Setup single axis Helmholtz coil system consisting of two identically wound and layered coils, connected in series
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 50 µT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Data demonstrated that ECTO-NOX-/CuII-catalyzed oscillations in NADH oxidation are phased by exposure to low frequency electromagnetic fields (i.e. they caused a phase shift).
The authors conclude that these data of electromagnetic field effects on phasing of the CuII-related oscillatory rhythm of ECTO-NOX proteins demonstrates for the first time a molecular mechanism whereby electromagnetic fields might be sensed by the time keeping machinery of cells.

Study character:

Study funded by

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