An aequorin expressing Arabidopsis thaliana mutant was subjected to a magnetic field around 65 µT and an electromagnetic field with the corresponding Ca2+ cyclotron frequency of 50 Hz. The applied ion cyclotron resonance (ICR) is one of the discussed mechanisms, predicting biological effects for definite frequencies and intensities of electromagnetic fields possibly by affecting the physiological availability of small ions.
The aequorin producing Arabidopsis mutant facilitates a powerful way to study the cytosolic Ca2+ flux in response to exogenic stressors.
|Exposure 1: 50 Hz|
|Exposure 2: 50 Hz|
|Exposure 3: 50 Hz|
The data suggest for the first time a direct and rapid influence of the resonant electromagnetic excitation of the cyclotronic frequency of Ca2+ on the concentration of this ion in the cytosol. This change was transient and relaxed within 60 minutes. Ca2+ transients were observed both by switching the Ca2+ ICR condition on and off.
Considering the various functions of Ca2+ as a second messenger in plants, this mechanism may be relevant for perception of these combined fields.