|Setup||two pairs of Helmholtz coils, one vertical, one horizontal; horizontal pair: radius of 0.09 m and 1000 turns, connected to an AC generator vertical pair: radius of 0.068 m and 100 turns, connected to a DC power supply and used to zero the ambient magnetic field; DC field parallel to the AC magnetic field; magnetic field in the exposure area homogenous within +/- 5 %|
|Sham exposure||A sham exposure was conducted.|
|Additional info||during sham exposure only the AC field was switched off in the middle of each experiment control samples were incubated at the location with the same DC magnetic field as in exposure/sham exposure|
The magnetic field exposures (5-20 µT) affected the chromatin conformation. These magnetic field effects differed significantly between the two donors and depended on magnetic flux density and initial condensation of chromatin. While the initial state of chromatin was rather stable in one donor (during different measurements), the initial condensation varied significantly in cells from another donor. Both this variation and the magnetic field effect depended on room temperature during exposure. Despite these variations, the general rule was that magnetic field exposure condensed the initially relaxed chromatin and relaxed the chromatin in cells with initially condensed chromatin. In other words, the initial chromatin state might determine both direction (condensation versus relaxation) and magnitude of the effects.
In conclusion, the data showed that individual effects of 50 Hz magnetic field exposure (5-20 µT) may be observed in human lymphocytes in dependence on the initial state of chromatin and temperature.