Study type: Medical/biological study (experimental study)

Histological, Ultrastructural and Immunohistochemical Studies of the Low Frequency Electromagnetic Field Effect on Thymus, Spleen and Liver of Albino Swiss Mice. med./bio.

Published in: Pak J Biol Sci 2002; 5 (9): 931-937

Aim of study (acc. to author)

To investigate the effects of extremely low frequency magnetic fields on the thymus, spleen and liver of mice.

Background/further details

Two groups of mice (control group and experimnetal) were used (n=10 per group).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 10, 20 or 50 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 10, 20 or 50 days
Exposure setup
Exposure source
  • not specified
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

While the tissue slices from the control group appeared normal, the histopathological examination revealed damages in the tissue from exposed mice, especially after 20 and 50 days of exposure:
In the thymus of exposed mice, a moderate loss of lymphocyte populations was found after 20 days, while pycnotic cells were found after 50 days. Moreover, the thymus tissue showed an increase in the enzyme activity of the glutathione S-transferase.
In the spleen of exposed mice, depletion of splenocytes and the presence of megakaryocytes were observed and lesions in the spleen tissue became more prominent when the exposure time was prolonged. Additionally, the enzyme activity of the glutathione S-transferase was gradually decreased due to the exposure time.
Also in the liver of exposed mice, the enzyme activity of the glutathione S-transferase decreased in a time-dependent manner. Ultrastructural examination of the liver from exposed mice revealed highly irregular nuclei, autolyzed chromatin and degenerated mitochondria.
The authors conclude that exposure to extremely low frequency magnetic fields could have a deleterious effect on the thymus, spleen and liver from mice.

Study character:

Study funded by

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