Study type: Medical/biological study (experimental study)

Occupational exposure to 50 Hz magnetic fields does not alter responses of inflammatory genes and activation of splenic lymphocytes in mice. med./bio.

Published in: Int J Occup Med Environ Health 2016; 29 (2): 277-91

Aim of study (acc. to author)

The effects of exposure of mice and splenic lymphocytes to a 50 Hz magnetic field on the immune function of the spleen should be investigated.

Background/further details

The study comprised an in vivo and an in vitro part. For the in vivo party, 20 mice were divided into 2 groups (n=10 each): 1) exposure (500 µT) and 2) sham exposure. For the in vitro part, splenic lymphocytes were isolated from 10 mice, stimulated with PMA (50 ng/ml) and ionomycin (1 µM) and divided into the following groups: exposure to a magnetic field with 3) 250 µT, 4) 500 µT and 5) 1 mT and 6) sham exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 8 hours/day on 5 days/week for 60 days
in vivo tests
Exposure 2: 50 Hz
Exposure duration: intermittent (?) for 6 hours
in vitro tests
Exposure 3: 50 Hz
Exposure duration: intermittent (?) for 6 hours
in vitro tests
Exposure 4: 50 Hz
Exposure duration: intermittent (?) for 6 hours
in vitro tests

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 8 hours/day on 5 days/week for 60 days
Additional info in vivo tests
Exposure setup
Exposure source
Chamber plastic cage
Setup solenoid was made of winding copper wire (Ø = 1 mm) with an internal diameter of 35 cm and length of 90 cm constructed on a glass fiber cylinder; uniformity of the MF in the exposure area was ± 3%; temperature was kept at 22 ± 2°C and humidity was kept at 55 ± 10% during exposure
Sham exposure A sham exposure was conducted.
Additional info for sham exposure, mice were kept in an animal room with "basal" exposure and additional stress like the exposure group, but without MF exposure, were used
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent (?) for 6 hours
Additional info in vitro tests
Exposure setup
Exposure source
Chamber dishes in incubator
Setup setup consisted of two 4-coil systems (2 coils with 56 windings, 2 coils with 50 windings), each placed inside a µ-metal box; both systems were fitted inside an incubator to ensure constant environmental conditions (37°C, 5% CO2, 95% humidity); temperature at the location of the dishes maintained 36.5-37.5°C during exposure; the temperature difference between the chambers did not exceed 0.3°C
Sham exposure A sham exposure was conducted.
Additional info for sham exposure, current in the coils was switched to non-parallel
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 250 µT - measured - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent (?) for 6 hours
Additional info in vitro tests
Exposure setup
Exposure source
  • same setup as in E2
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT - measured - -

Exposure 4

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent (?) for 6 hours
Additional info in vitro tests
Exposure setup
Exposure source
  • same setup as in E2
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

After 20 and 30 days, exposed mice (group 1) had a significantly lower body weight than sham exposed mice (group 2) (remark EMF-Portal: result not clear, contradiction between text and figure). All other parameters did not show any significant differences between exposure and sham exposure groups of mice and cells.
The authors conclude that exposure of mice and splenic lymphocytes to a 50 Hz magnetic field has no effects on the immune function of the spleen.

Study character:

Study funded by

Related articles